| Summary: | 碩士 === 國立成功大學 === 奈米科技暨微系統工程研究所 === 96 === The accurate result can be provided with the conventional detection of microorganism, but the procedures took more time and cost. To develop the fast and accurate detection system is very important for the diagnosis of pathogens. Among these techniques, dielectrophoretic (DEP) chip is a common method for the application of bio-particles manipulation. In classical DEP theories, it describes that the non-uniform electric field made by metallic electrode alignment product the polarization in the solid-liquid interface and it make dielectric particles to move the high or low intensity regions of electric fields, and then to be manipulated and separated.
It is important to research the proper conditions of trapping bio-particles in my experiment. If the samples are latex beads, we use DI water for medium, and the voltage of 20 Vp-p, frequency of 10 MHz and fluid velocity of 0.75~ 1.25 mm/s, respectively. The trapping efficiency can be above 90%. If the samples are microbes, we use PBS buffer mixed D-mannitol solution for the medium, and the voltage of 20 Vp-p, frequency of 500 kHz and fluid velocity of 0.75 mm/s, respectively. The trapping efficiency of E. faecium can be above 85%. The concentration tended to be stable after trapping microbes for 6 minutes. To compare with other conventional detection techniques, like the turbidity meter and plate counting procedures, the concentrations are close. Applications for the 3D DEP chip will include the quantification of microbes in foodstuffs or environment. According to the results, this chip can be promoted to be a lab-on-a-chip in the future.
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