| Summary: | 碩士 === 國立嘉義大學 === 動物科學系研究所 === 96 === Stem cells are capable of self-renew and differentiation to functional mature cells. There are at least three types of stem cells found in skin including epidermal stem cells, bulge stem cells, and dermis derived stem/progenitor cells. Present study was to investigate the multipotential differentiation plasticity of dermis derived stem/progenitor cells isolated from mouse fetal skin. In the current work, two digestion methods have been used to isolate dermal stem/progenitor cells. The first method involved two stages of digestion by utilizing collagenase type I and trypsin, and the second digestion methods used trypsin only. Basically, we found that the second method can be utilized to efficient isolate nestin-expressing dermal stem/progenitor cells. We also revealed dermal stem/progenitor cells isolated embryo day 15.5 (E15.5) mouse skin had the highest sphere-forming ability and expressed neural crest marker such as sox9 and slug. When these dermal stem/progenitor cells were grown on poly-D-lysine and laminin coating slide, serum-containing medium can induce these cells to differentiate into neuron, astrocyte, and fibroblast-like cells. In contrast, if dermal stem/progenitor cells were cultured in medium containing dexamethasone, insulin and fetal bovine serum for 3 days, these cells could become adipocytes. And DSCs were cultured in medium containing HGF and bFGF for 7 days, these cells can differentiate into hepatocyte precursor cells. In summary, the current study demonstrates that stem/progenitor cells isolated from fetal mouse dermis possibly have the multipotency to differentiate into mesodermal, and ectodermal lineages, and DSC have the potential for differentiation into hepatocytes (endodermal).
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