| Summary: | 碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 96 === The purpose of study is investigated to measure the cell membrane stiffness for the red blood cells of anemia and normal rats using AFM. On the other hand, an triangle micro-structure chip is made by micro-manufacture technology then the non-uniform electric field which is worked on the DC pulse voltage to destroy the red blood cells of anemia and normal rats. This study investigated the relationships among cell membrane stiffness, cell lysis time and physiology conditions for the red blood cells of rats.
From the experimental results, that the membrane stiffness of anemia red blood cell, 110.909x10-3N/m, is significantly higher than that of normal red blood cell, 52.785 x10-3N/m. Then the physical conditions of RBC, HGB, HCT in the anemia are decreased significantly, both of them can be used to determine whether the basis for anemia. It means the membrane stiffness of anemia cell increased as the physical conditions of RBC, HGB, HCT decrease. However, the relationship between the cell lysis time of red blood cells and membrane stiffness or physiological conditions is no obvious significance. It is not possible to the cell lysis time of red blood cells whether or not the basis for anemia.
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