Differential display of nervous necrosis virus (nodavirus) and iridovirus infected grouper cells by immunostaining approach

• Main Authors: Chiao-Hwa Yeh, 葉椒華
• Other Authors: Nin-Nin Chuang
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/23517086226710593673

博士 === 國防醫學院 === 生命科學研究所 === 96 === Grouper iridovirus (GIV) and nervous necrosis virus (NNV) are two of the most devastating infectious pathogens of aquaculture fish. When infecting the susceptible cell lines, such as GK-2 from kidney and GB3 from brain, GIV and NNV can cause antigenic changes in host cellular proteins respectively. To understand the host gene expression characteristics after viral infection, we developed an immunostaining method to screen differentially expressed genes of fish cells in response to GIV and NNV infection using phage display complementary DNA libraries. In total, 66 and 67 genes were identified from grouper kidney and brain cell lines responded to GIV and NNV infection. In which, 63 genes were in common. These genes are related to replication, transcription, translation, immunity, apoptosis, structure proteins, metabolism, energy, protein modification, and homeostasis. Four dynamic antigenic patterns were observed among these immunocloned genes upon virus infection. Microarray analysis further confirmed the transcriptional patterns of 80% of the identified genes. This immunostaining screening method provides insights into a host's cellular protein response to viral infection on a translational basis.