The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions
碩士 === 國防醫學院 === 生物化學研究所 === 96 === Glucocorticoid receptor-interacting protein 1 (GRIP1), one of the p160 family co-activator proteins, primarily mediates transcriptional activation by nuclear receptors via three activation domains that recruit at least three secondary co-activators, CBP/p300, co-a...
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ndltd-TW-096NDMC01070162016-05-16T04:09:52Z http://ndltd.ncl.edu.tw/handle/97625255370599833518 The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions 探討GRIP1羧端輔因子功能的調控因子 Shu-Ting Liu 劉淑婷 碩士 國防醫學院 生物化學研究所 96 Glucocorticoid receptor-interacting protein 1 (GRIP1), one of the p160 family co-activator proteins, primarily mediates transcriptional activation by nuclear receptors via three activation domains that recruit at least three secondary co-activators, CBP/p300, co-activator-associated arginine methyltransferase 1, and coiled-coil co-activator (CoCoA) which exert histone acetyltransferase and/or arginine methyltransferase enzyme activity. The regulatory mechanisms underlying the GRIP1 co-activation functions involve its post-translational sumoylation and ubiquitin-conjugating enzyme 9 (UBC9), but are not well understood. This thesis demonstrates that promyelocytic leukaemia protein (PML) and UBC9 specifically act on the C-terminal transactivation activity of GRIP1 using the GRIP1 N-terminal co-activator, CoCoA, and some truncated GRIP1 fragments. The mechanisms of enhancement by PML and UBC9 both suppressed the repressive effect within the GRIP1 C-terminal region depended on the sumoylation status of PML, but not on the sumo-conjugating enzyme activity of UBC9. Daxx is not the primary target of PML for the regulation of GRIP1 co-activator functions, whereas, PML-RAR has the ability to suppress the enhancement of PML on GRIP1, mediating the truth of the hetero-dimerization of PML and PML-RAR. The activities of PML and UBC9 might be involved in the regulation of GRIP1 transactivation activity to enhance the nuclear receptor co-activator functions of GRIP1. This thesis provides new insights into the sumoylation status, the common C-terminal structure of PML family proteins, and the nuclear localization signal of PML in the regulation of the GRIP1 co-activator functions. Shih-Ming Huang 黃世明 2008 學位論文 ; thesis 69 zh-TW |
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碩士 === 國防醫學院 === 生物化學研究所 === 96 === Glucocorticoid receptor-interacting protein 1 (GRIP1), one of the p160 family co-activator proteins, primarily mediates transcriptional activation by nuclear receptors via three activation domains that recruit at least three secondary co-activators, CBP/p300, co-activator-associated arginine methyltransferase 1, and coiled-coil co-activator (CoCoA) which exert histone acetyltransferase and/or arginine methyltransferase enzyme activity. The regulatory mechanisms underlying the GRIP1 co-activation functions involve its post-translational sumoylation and ubiquitin-conjugating enzyme 9 (UBC9), but are not well understood. This thesis demonstrates that promyelocytic leukaemia protein (PML) and UBC9 specifically act on the C-terminal transactivation activity of GRIP1 using the GRIP1 N-terminal co-activator, CoCoA, and some truncated GRIP1 fragments. The mechanisms of enhancement by PML and UBC9 both suppressed the repressive effect within the GRIP1 C-terminal region depended on the sumoylation status of PML, but not on the sumo-conjugating enzyme activity of UBC9. Daxx is not the primary target of PML for the regulation of GRIP1 co-activator functions, whereas, PML-RAR has the ability to suppress the enhancement of PML on GRIP1, mediating the truth of the hetero-dimerization of PML and PML-RAR. The activities of PML and UBC9 might be involved in the regulation of GRIP1 transactivation activity to enhance the nuclear receptor co-activator functions of GRIP1. This thesis provides new insights into the sumoylation status, the common C-terminal structure of PML family proteins, and the nuclear localization signal of PML in the regulation of the GRIP1 co-activator functions.
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author2 |
Shih-Ming Huang |
author_facet |
Shih-Ming Huang Shu-Ting Liu 劉淑婷 |
author |
Shu-Ting Liu 劉淑婷 |
spellingShingle |
Shu-Ting Liu 劉淑婷 The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions |
author_sort |
Shu-Ting Liu |
title |
The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions |
title_short |
The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions |
title_full |
The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions |
title_fullStr |
The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions |
title_full_unstemmed |
The study of regulatory factors for the C-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (GRIP1) functions |
title_sort |
study of regulatory factors for the c-terminal coactivation domain of glucocorticoid-receptor- interacting-protein 1 (grip1) functions |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/97625255370599833518 |
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