Different Pretreatment of Agricultural Waste in the Hydrolsis of Cellulose Enzyms – An example of Pinapple Leaf Cellulose

碩士 === 國立屏東科技大學 === 生物機電工程系所 === 96 === Taiwan's agricultural waste about 5 million tonnes of annual output, a huge number of agricultural wastes make a great impact to the environment. At present, farmers still use the traditional burial or burning as waste disposal method. It’s necessary to c...

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Bibliographic Details
Main Authors: Rong-Bin Lin, 林榮斌
Other Authors: Pomin Li
Format: Others
Language:zh-TW
Published: 2008
Online Access:http://ndltd.ncl.edu.tw/handle/75048785074960787681
Description
Summary:碩士 === 國立屏東科技大學 === 生物機電工程系所 === 96 === Taiwan's agricultural waste about 5 million tonnes of annual output, a huge number of agricultural wastes make a great impact to the environment. At present, farmers still use the traditional burial or burning as waste disposal method. It’s necessary to create a new solution for the increasing agricultural waste. If these agricultural wastes can be transformed into resources, it will not only reduce the agricultural waste but also generate the new resources. Biotechnology can be used in a wide area on agriculture waste. The most common method is chemical pretreatment method, it can the reducing sugars from the wood fiber quickly, besides it makes a great enhancement to the renewable energy of agricultural waste. In this study, it use the common agricultural waste(Pineapple leaves, rice-and-banana, bagasse)from Pingtung areas as raw materials. Sulfuric acid and alkali uas catalyst. Concentrated sulphuric acid to 72 percent and hydrolyze it at 30 ℃ for two hours, and then hydrolyze it at 121℃. Do the pre-processing to the dilute acid and alkali at different temperatures (60, 90,120℃), different concentrations (0.5, 1, 1.5 M) and different processing time (20, 40, 60 min) then analysis the percentage of reducing sugar from it. The results showed after compared the control group (CG) and experimental group (EG), it generates more than 3mg reducing suger in EG and only less than 1 mg or 1 mg in control group, Acid hydrolysis in the pre-treatment temperature and time and concentration, with the temperature and the concentration of reducing sugar concentration gradually increased, in 1 M and 1.5 M. before the temperature 90 and 120 pre-treatment at reducing sugar produced 13.2 mg and 13.1 mg No significant differences to alkaline. After 1 M. before the temperature at 90℃, 60 min time reducing sugar under the dissolution rate higher. Hydrolysis enzymes in the alkali treatment to deal with concentrations of 1.5 M up to the highest average conversion rate of over 12 percent. In pre-treatment in the enzyme acid hydrolysis results were slightly raise the average conversion rate of around 7.8% in.