The Characteristic Analysis of DGNNV Virus-Like Particles
碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === Fish nodavirus causes the death of several high economic fish. For studying the package and stability of dragon grouper necrosis virus (DGNNV), wild type virus-like particles (wt-VLPs) and ΔN25-VLPs were employed. After experiments of disassembly and assem...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2007
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| Online Access: | http://ndltd.ncl.edu.tw/handle/89b3d9 |
| Summary: | 碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === Fish nodavirus causes the death of several high economic fish. For studying the package and stability of dragon grouper necrosis virus (DGNNV), wild type virus-like particles (wt-VLPs) and ΔN25-VLPs were employed. After experiments of disassembly and assembly, the result showed that DNA of 608 bp was able to be packaged by wt-VLPs. The sedimentation of VLPs was affected by different pH buffers during the process of purification: the VLPs at alkaline conditions behaved faster than those at the acidic. The stability of pure VLPs was pH-independent. The micrographs of the wt-VLPs in alkaline buffers showed that particles were rough and irregular in shapes, some of which were stain-permeable. However, the wt-VLPs in acidic buffers were morphologically indistinguishable from the untreated VLPs. The Western blotting for gradiently purified miture of VLPs and lysozyme revealed that lysozyme was co-precipitated with wt-VLPs.
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