The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === Cyro-EM and three-dimensional reconstruction have become important research tools for virus structure. These techniques have benefit of fast and keep samples in native folding. Simulated prefer orientation images of DGNNV, PaV and TBSV were reconstructed b...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2008
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| Online Access: | http://ndltd.ncl.edu.tw/handle/f7f259 |
| Summary: | 碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === Cyro-EM and three-dimensional reconstruction have become important research tools for virus structure. These techniques have benefit of fast and keep samples in native folding. Simulated prefer orientation images of DGNNV, PaV and TBSV were reconstructed by SPIDER or PurdueEM, with projection matching with. SPIDER prefers 3-fold and 5-fold view fields, and PurdueEM prefers 2-fold view fields. SPIDER could reconstruction images which have more noise than PurdueEM can successfully reconstruct. Reconstruction of RNA-cages has some
relationship with the symmetry of capsid protein. Prefer orientation, noise and RNA-cages are the factors that can effect reconstruction.
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