The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles

碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === Cyro-EM and three-dimensional reconstruction have become important research tools for virus structure. These techniques have benefit of fast and keep samples in native folding. Simulated prefer orientation images of DGNNV, PaV and TBSV were reconstructed b...

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Main Authors: Chun-Hong Chen, 陳俊宏
Other Authors: Chan-Shing Lin
Format: Others
Language:zh-TW
Published: 2008
Online Access:http://ndltd.ncl.edu.tw/handle/f7f259
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spelling ndltd-TW-096NSYS52700132018-05-13T04:29:00Z http://ndltd.ncl.edu.tw/handle/f7f259 The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles 視角取向對T=3病毒顆粒三維重建之影響 Chun-Hong Chen 陳俊宏 碩士 國立中山大學 海洋生物科技暨資源學系研究所 96 Cyro-EM and three-dimensional reconstruction have become important research tools for virus structure. These techniques have benefit of fast and keep samples in native folding. Simulated prefer orientation images of DGNNV, PaV and TBSV were reconstructed by SPIDER or PurdueEM, with projection matching with. SPIDER prefers 3-fold and 5-fold view fields, and PurdueEM prefers 2-fold view fields. SPIDER could reconstruction images which have more noise than PurdueEM can successfully reconstruct. Reconstruction of RNA-cages has some relationship with the symmetry of capsid protein. Prefer orientation, noise and RNA-cages are the factors that can effect reconstruction. Chan-Shing Lin 林全信 2008 學位論文 ; thesis 121 zh-TW
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language zh-TW
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description 碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === Cyro-EM and three-dimensional reconstruction have become important research tools for virus structure. These techniques have benefit of fast and keep samples in native folding. Simulated prefer orientation images of DGNNV, PaV and TBSV were reconstructed by SPIDER or PurdueEM, with projection matching with. SPIDER prefers 3-fold and 5-fold view fields, and PurdueEM prefers 2-fold view fields. SPIDER could reconstruction images which have more noise than PurdueEM can successfully reconstruct. Reconstruction of RNA-cages has some relationship with the symmetry of capsid protein. Prefer orientation, noise and RNA-cages are the factors that can effect reconstruction.
author2 Chan-Shing Lin
author_facet Chan-Shing Lin
Chun-Hong Chen
陳俊宏
author Chun-Hong Chen
陳俊宏
spellingShingle Chun-Hong Chen
陳俊宏
The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
author_sort Chun-Hong Chen
title The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
title_short The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
title_full The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
title_fullStr The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
title_full_unstemmed The Effects of Prefer Orientation on Three-Dimensional Reconstruction of T = 3 Virus Particles
title_sort effects of prefer orientation on three-dimensional reconstruction of t = 3 virus particles
publishDate 2008
url http://ndltd.ncl.edu.tw/handle/f7f259
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