| Summary: | 碩士 === 國立臺南大學 === 材料科學系碩士班 === 96 === Clinopodium laxiflorum including two varieties : laxiflorum var. laxiflorum and laxiflorum var. taiwanianum, is an endemic species in Taiwan. In this study, we sampled 11 populations including 94 individuals and completed the systematic of Clinopodium, the genetic diversity and phylogeography of C. laxiflorum from chloroplast DNA ( 2 non-coding region: atpB-rbcL, trnL-trnF), The chloroplast DNA atpB-rbcL non-coding region had 693 bp (base pair) after alignment, with 12 polymorphic sites, 0.00006 value of nucleotide diversity, 0.37 value of haplotype diversity. The chloroplast DNA trnL-trnF non-coding region had 281 bp (base pair) after alignment, with 14 polymorphic sites, 0.00226 value of nucleotide diversity, 0.55 value of haplotype diversity. Based on the analysis of 2 chloroplast DNA regions, the common haplotype of C. laxiflorum was distributed in most populations. Under neighbor-joining analysis, C. chinense was inner clade, and separated with C. laxiflorum obviously. Under genealogical analysis, there is closely relationship between haplotypes of C. chinense and C. laxiflorum. Estimating coalescent time, the differentiated time of C. chinense and C. laxiflorum was about 0.9~1.3 million years ago in the postglacial of Gunz (1.2~2.0 million years ago). Since the nucleotide diversity and haplotype diversity were in low level, it might be the bottleneck effect during the glacial period in the past.
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