| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 96 === Abstract
Corbicula fluminea, also known as fresh water clam, is an important cultured bivalve in Taiwan. In recent years, many reports have found that Corbicula fluminea has some bioactivities, including antioxidant, anti-hypertension, anti-cancer and hepatoprotective activities. The aims of this study were to isolate bioactive compounds and establish HPLC profile.
The bioactive compounds of Corbicula fluminea were evaluated based on lipid peroxidation induced by CCl4 or hemoglobin. The results showed that the fraction FE-HCa has higher anti-oxidative activity. After further purification, the anti-oxidative activity of FE-HCa-I2P2 and FE-HCa-I3P2 were 19.90% and 16.33%, and their inhibitory activities against CYP2E1 were 62.8% and 64.1%, respectively. FE-HCa-I4P1 was a known compound, di-(2-ethylhexyl) phthalate, based on NMR, HPLC and MS, was an environmental hormone usually found in the marine organism. It was knowed that FE-FEe-4 has good inhibitory on cell viability of Hep G2, and its pure compound FE-FEe-4P1 is a sterol with twenty-nine carbons.
Two bioctive compounds of Corbicula fluminea, 3,5,9-trihydroxy -cholest-7-en-6-one and 3,7,9-trihydroxy-cholest-5-ene, and two synthesized ergosterol peroxide and cholesterol peroxide, were used as standards to establish the HPLC analysis. By using HPLC and TLC, it is found that ergosterol peroxide, cholesterol peroxide, 3,5,9-trihydroxy- cholest-7-en-6-one and 3,7,9-trihydroxy-cholest-5-ene were present in the fractions FE-BDb, FE-BEc and FE-BHc.
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