The Model of Acetaminophen-induced Liver Damage inMice and the Hepatoprotective Effect of Ziziphus jujubaMill. on Liver Damage in Mice

• Main Authors: Hsiao-Yin Chou, 周筱胤
• Other Authors: 沈立言
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/16162641851607696921

碩士 === 國立臺灣大學 === 食品科技研究所 === 96 === Liver cancer is the second cause of death in malignant neoplasms in 2007 in Taiwan. Chronic liver disease and cirrhosis are the seventh of the top ten causes of death. Therefore, hepatoprotection is very important for Taiwanese. Nowadays, carbon tetrachloride (CCl4)-induced hepatotoxicity is used to evaluate the hepatoprotective effect on Health Foods (Department of Health, Executive Yuan, Taiwan, R.O.C., 2007).However, CCl4-induced liver damage is not common to people. In the recent studies, acetaminophen (APAP)-induced hepatotoxicity was used to evaluate some foods which have anti-oxidative or hepatoprotective effect. The first aim of this study was to establish the model of APAP-induced liver damage in mice. The results indicated that the appropriate doses to induce acute and chronic liver damage in ICR mice or BALB/c mice both are APAP 600 and 400 mg/kg bw, respectively. We choose BALB/c mice to be the animal model for inducing liver damage. That’s because the survival rate of BALB/c mice is better than ICR mice. Another aim of my study is to evaluate the hepatoprotective effect of Ziziphus jujuba Mill. (ZJ) using this animal model. It is commonly used for keeping human in good health, rengthening qi (氣), nourishing the blood (養血) and harmonizing the functions of different drugs (中和百藥). The results indicated that the administration of ZJ (3, 15, 30 g/kg bw) decreased APAP-induced increases in aspartate aminotransferase (ALT), alanine aminotransferase (AST) and thiobarbituric acid-reactive substances (TBARS) values. ZJ could increase hepatic glutathione (GSH) contents, anti-oxidative enzymes glutathione peroxidase (GPx), glutathione reductase (GRd), superoxide dismutase (SOD) and catalase (CAT) activities.ZJ also could modulate the enzymes of hepatic detoxification system glutathione S-transferase (GST) and cytochrome P450 2E1 (CYP2E1). The hepatoprotective effect of ZJ was also confirmed in histopathological examination of the liver. In conclusion, the model of APAP-induced liver damage in BALB/c mice may be useful to evaluate the hepatoprotective effect on Health Foods, and ZJ may have the potential with hepatoprotective effect in this animal model.