Diversity and genetic structure among coffee populations in Taiwan revealed by RAPD molecular markers
碩士 === 國立臺灣大學 === 農藝學研究所 === 96 === The RAPD (Random Amplified Polymorphic DNA) markers were used to analyze the Taiwan coffee germplasm for understanding the genetic diversity and population structure. Eleven coffee accessions from the participant of the 2006 Taiwan Coffee Festival were used in thi...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2008
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| Online Access: | http://ndltd.ncl.edu.tw/handle/66742949121144313699 |
| Summary: | 碩士 === 國立臺灣大學 === 農藝學研究所 === 96 === The RAPD (Random Amplified Polymorphic DNA) markers were used to analyze the Taiwan coffee germplasm for understanding the genetic diversity and population structure. Eleven coffee accessions from the participant of the 2006 Taiwan Coffee Festival were used in this research. One hundred and ten blended DNA samples from the eleven coffee accessions were able to produce 170 unambiguous bands by 11 RAPD primers. One hundred and seven polymorphic bands were chosen for estimating the genetic diversity. The estimated genetic similarities between accessions were ranging from 67% to 97%. The cluster analysis based on the polymorphic bands using UPGMA methodology reveal similar result as the data analyzed by principle coordinate analysis. The average similarity between accessions was estimated to be 75% and not able to distinguished from one another other than the Nantou and Yunlin accessions. The result presented an evidence of narrow genetic diversity of the current coffee germplasm. Input of foreign germplasm should be required to broaden the genetic base of local germplasm.
The records from local historical coffee introduction documentation provided an explanation to the great similarity estimated by this research. The similarity between the accessions could be due to the spreading the coffee germplasm during the Japanese occupation.
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