The Synthesis and Characterization of Chitosan/Poly(caprolactone) Copolymer

• Main Authors: Po-Sheng Huang, 黃博聖
• Other Authors: Wen-Jen Lin
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/15688481956876801976

碩士 === 國立臺灣大學 === 藥學研究所 === 96 === The development of drug delivery systems were going well, and the releated researches were very numerous in the past few decade. The resources of materials were having two characteristics which were biocompatible and biodegradable. We attempted to synthesize a new amphiphillc graft copolymer, mPEG-g-DADP-CS-g-PCL. The all part of materials were non-toxic. Chitosan is very abundant material in the world, poly(ε-caprolactone) and mPEG are the materials that already approved by FDA. In this research, chitosan was depolymerized until it can dissolve in the water, where the Mn was around 2000 g/mol. Each sample was identified by IR and NMR, and the thermal properties by DSC. The CMC of this new material was measured by using pyrene. Indomethaicn and theophylline were chosen as the model drugs to evaluate the drug loading and loading efficiency in the micelles. The interaction of the drug and micelles were measured by NMR. Finally, the surface charge and size distribution of complex of TPP and graft copolymer were investigated. The properities of three graft copolymers, mPEG-g-DADP-CS-g-PCL, were listed below: 1、 The size distributions were 124±9.31nm(#10), 563.57±54.11nm(#11-12), and 206.53±9.28nm(#13). 2、 The surface charge of this three polymers were 3±0.2mV(#10), 2.57±0.32mV(#11-12), and 2.80±0.17mV(#13). 3、 The entrapment of efficiency(%) of indomethacin were 38.58±2.6%(#10), 38.19±7.37%(#11-12), and 23.70±3.04%(#13). 4、 The entrapment of efficiency(%) of theophylline were 10.28±5.65%(#11-12) and 2.08±0.09%(#13). 5、 The unloaded micelles were very stable during a few weeks. 6、 The surface charge of the complex of the TPP and graft copolymer was reaching -20 to -30mV, and the size was higher than without TPP. Both depended on the concentration of polymer and TPP.