Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast
碩士 === 國立臺灣科技大學 === 化學工程系 === 96 === The engineering of cell surface display is immobilization of heterogeneous protein on cell surface . It is widely used in catalytic reaction of cell , adsorbent of cell , vaccine , biosensor .We discuss that we will display �毢eta-glucosidase on yeast surface and...
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ndltd-TW-096NTUS50630302016-05-13T04:15:16Z http://ndltd.ncl.edu.tw/handle/85620090627801367724 Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast 建構能分解纖維素寡糖之酵母菌發酵生產酒精之研究 CHENG-YU LIU 劉政佑 碩士 國立臺灣科技大學 化學工程系 96 The engineering of cell surface display is immobilization of heterogeneous protein on cell surface . It is widely used in catalytic reaction of cell , adsorbent of cell , vaccine , biosensor .We discuss that we will display �毢eta-glucosidase on yeast surface and use it to produce bioethanol from cellulose.Beta-glucosidase can hydrolyzes cellobiose to glucose that can provide energy for biomass and producing ethanol. 100(g/l) cellobiose as carbon source can obtain 23(g/l) ethanol from Beta-glucosidase-surface displaying yeast , but host can’t produce any ethanol , therefore the technology is opportunity to directly utilize cellulose to produce ethanol by simultaneous saccharification and fermentation (SSF) . Although commercial cellulose from Trichoderma contain endogenous Beta-glucosidase , but the activities of this enzyme are generally insufficient to prevent the accumulation of cellobiose, resulting in product inhibition of endoglucanases and cellobiohydrolases. Therefore we tried genetically to immobilize Beta-glucosidase on the cell surface of the yeast Saccharomyces cerevisiae in its active form to supply this insufficient enzyme , and then successfully promote the efficiency of producing ethanol from cellulose by SSF process . Cheng-Kang Lee 李振綱 2008 學位論文 ; thesis 102 zh-TW |
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碩士 === 國立臺灣科技大學 === 化學工程系 === 96 === The engineering of cell surface display is immobilization of heterogeneous protein on cell surface . It is widely used in catalytic reaction of cell , adsorbent of cell , vaccine , biosensor .We discuss that we will display �毢eta-glucosidase on yeast surface and use it to produce bioethanol from cellulose.Beta-glucosidase can hydrolyzes cellobiose to glucose that can provide energy for biomass and producing ethanol. 100(g/l) cellobiose as carbon source can obtain 23(g/l) ethanol from Beta-glucosidase-surface displaying yeast , but host can’t produce any ethanol , therefore the technology is opportunity to directly utilize cellulose to produce ethanol by simultaneous saccharification and fermentation (SSF) . Although commercial cellulose from Trichoderma contain endogenous Beta-glucosidase , but the activities of this enzyme are generally insufficient to prevent the accumulation of cellobiose, resulting in product inhibition of endoglucanases and cellobiohydrolases. Therefore we tried genetically to immobilize Beta-glucosidase on the cell surface of the yeast Saccharomyces cerevisiae in its active form to supply this insufficient enzyme , and then successfully promote the efficiency of producing ethanol from cellulose by SSF process .
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author2 |
Cheng-Kang Lee |
author_facet |
Cheng-Kang Lee CHENG-YU LIU 劉政佑 |
author |
CHENG-YU LIU 劉政佑 |
spellingShingle |
CHENG-YU LIU 劉政佑 Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
author_sort |
CHENG-YU LIU |
title |
Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
title_short |
Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
title_full |
Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
title_fullStr |
Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
title_full_unstemmed |
Ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
title_sort |
ethanol fermentation from cellulose hydrolysate by a recombinant cellooligosaccharide-assimilating yeast |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/85620090627801367724 |
work_keys_str_mv |
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