Human Exposure Study of New Insect Repellent（Icaridin）

• Main Authors: Yu-fen Chang, 張玉芬
• Other Authors: Lih-Ming Yiin
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/07983583052806954026

碩士 === 慈濟大學 === 公共衛生研究所 === 96 === Background: There are many mosquito-borne diseases spread around the world, and they have great impacts upon people’s health. The dengue fever is one of the diseases prevalent in south Taiwan. Thus, the Taiwan government is promoting the mosquito control and the knowledge of vector-borne diseases which are known to people. Besides, the advertisement of insect repellent enriches people’s knowledge to choose insect repellent to protect themselves from mosquito bites. DEET and those based on natural formula are the main active ingredients of most commercial insect repellents. Studies showed that the permeation of DEET and oxybenzone-containing sunscreen across the piglet skin resulted in synergistic absorption of both. This report raised a health concern about using both products simultaneously. Icaridin is a new active ingredient of insect repellent, and whether it has the synergistic effect while being used with oxybenzone is yet to be studied. Therefore, this research was to find out the transdermal permeation of Icaridin and oxybenzone by using the Franz diffusion cells. Purpose: 1. to establish the in vitro transdermal permeation of Icaridin; 2. to compare the permeation of DEET and Icaridin; 3. to examine the effect of oxybenzone on the permeation of Icaridin; 4. to examine the impact of solvents on permeation. Methods: The in vitro transdermal permeation study as conducted using Franz cells and piglet skins. The receptor fluid was phosphate buffer solution containing 6％ Brij solution. The donor compounds included 1) 0.5ml Icaridin solutions (including commercial product, pure Icaridin and 12％ Icaridin solution ) 2) 0.5ml DEET solutions (including commercial product, pure DEET and 12％ DEET solution ) 3) mixture of 0.5ml Icaridin solutions (including commercial product and 12％ Icaridin solution ) and 0.5ml oxybenzone solution (including commercial product and 3.8％ oxybenzone solution ). Results: The permeation of DEET was significantly higher than that of Icaridin（p<0.05）. The solvent effect was significantly on the permeation of drug, especially the permeation of DEET in 40% isoproponal solution was significantly higher than others. The solvent effect on DEET in 40% isoproponal solution was significantly higher than that on Icaridin in 40% isoproponal solution（p<0.001）. The permeation percentage of mixture solution of Icaridin and oxybenzone (bottom: oxybenzone) was significantly higher than that of lone Icaridin（p<0.05）. Conclusion: The transdermal permeation of Icaridin was less than that of DEET. The permeation of Icaridin was increased with the presence of oxybenzone, and depended upon the application sequence.