Establishing A Liver Regeneration Model in Zebrafish: The Role of Fgf Signaling

• Main Authors: Yin-ju Chen, 陳盈如
• Other Authors: Wen-Pin Wang
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/n7u46k

碩士 === 慈濟大學 === 分子生物及細胞生物研究所 === 96 === Liver has an unusual capacity to regenerate after a variety of injuries. In contrast to the deregulated cell division in liver disease, liver regeneration is a tightly controlled process. Zebrafish is a good model for forward and reverse genetic studies. We are interested in studying the mechanism of zebrafish liver regeneration, so we use zebrafish to study this process. We have established a liver regeneration model by partial hepatectomy (PH) in zebrafish. Then, we analyzed gene expression profiles, histological architecture, metabolic function, and cell proliferation in the processes. In this study, cell proliferation detected by PCNA and BrdU staining was increased at 3 dpa after PH. Furthermore, RT-PCR, microarray, and section in situ hybridization were performed to screen the gene expression during liver regeneration. The regenerative marker Msxb and several Fgfs, Fgfr2c and Fgfr4 were up-regulated during liver regeneration. Since Fgf signaling is participated in the various regenerations and it also plays an important role of liver development in zebrafish, we hypothesize that fgfs may have an important role in zebrafish liver regeneration. Furthermore, we showed that either fgfr inhibitor SU5402 or hsp70:dnfgfr-egfp fish could inhibit the proliferation and induce apoptosis of 3dpa regenerating liver. These results demonstrated that FGF signaling was important for liver regeneration and it affected hepatocyte proliferation and survival in the process. The interactions of fgf and other signaling pathways will also be addressed in the processes.