Circular Dichroism and ESR Studies of Peptide Derivatives
碩士 === 東海大學 === 化學系 === 96 === This thesis is divided into two major parts. The first part reports a circular dichroism (CD) study to unveil the cooperativity in 11-mer duplex DNA bearing single 5’-d(AAAA)-d(TTTT)-3’ or 5’-d(AAA)-d(TTT)-3’ binding sites. CD studies show that peptides RPyH-12 and RY...
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ndltd-TW-096THU000650162015-11-30T04:02:53Z http://ndltd.ncl.edu.tw/handle/78699853495394886028 Circular Dichroism and ESR Studies of Peptide Derivatives 胜肽衍生物之圓二色光譜及電子共振光譜研究 Tiffany Lin 林筱筑 碩士 東海大學 化學系 96 This thesis is divided into two major parts. The first part reports a circular dichroism (CD) study to unveil the cooperativity in 11-mer duplex DNA bearing single 5’-d(AAAA)-d(TTTT)-3’ or 5’-d(AAA)-d(TTT)-3’ binding sites. CD studies show that peptides RPyH-12 and RY-12 bind strongly to the duplex with positive cooperativity. Incubation temperature is shown to have a marked effect on observed cooperativity (Hill coefficient). The second part reports Electron Paramagnetic Resonance (EPR) studies on several new 2,6-dimethoxyhydroquinone-dipeptide conjugates that give strong EPR signals in aqueous solution containing ferrous sulfate. The facile generated semiquinone radicals are considered to be responsible for observed DNA cleavage. These new 2,6-dimethoxyhydroquinone-dipeptide conjugates should be useful in future semiquinone footprinting studies. This thesis also reports the synthesis of five new peptides bearing the XPRK motif that may be useful ligands for DNA molecular recognition studies. L. Sheh 佘亮 2008 學位論文 ; thesis 140 zh-TW |
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碩士 === 東海大學 === 化學系 === 96 === This thesis is divided into two major parts. The first part reports a circular dichroism (CD) study to unveil the cooperativity in 11-mer duplex DNA bearing single 5’-d(AAAA)-d(TTTT)-3’ or 5’-d(AAA)-d(TTT)-3’ binding sites. CD studies show that peptides RPyH-12 and RY-12 bind strongly to the duplex with positive cooperativity. Incubation temperature is shown to have a marked effect on observed cooperativity (Hill coefficient).
The second part reports Electron Paramagnetic Resonance (EPR) studies on several new 2,6-dimethoxyhydroquinone-dipeptide conjugates that give strong EPR signals in aqueous solution containing ferrous sulfate. The facile generated semiquinone radicals are considered to be responsible for observed DNA cleavage. These new 2,6-dimethoxyhydroquinone-dipeptide conjugates should be useful in future semiquinone footprinting studies.
This thesis also reports the synthesis of five new peptides bearing the XPRK motif that may be useful ligands for DNA molecular recognition studies.
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L. Sheh |
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L. Sheh Tiffany Lin 林筱筑 |
author |
Tiffany Lin 林筱筑 |
spellingShingle |
Tiffany Lin 林筱筑 Circular Dichroism and ESR Studies of Peptide Derivatives |
author_sort |
Tiffany Lin |
title |
Circular Dichroism and ESR Studies of Peptide Derivatives |
title_short |
Circular Dichroism and ESR Studies of Peptide Derivatives |
title_full |
Circular Dichroism and ESR Studies of Peptide Derivatives |
title_fullStr |
Circular Dichroism and ESR Studies of Peptide Derivatives |
title_full_unstemmed |
Circular Dichroism and ESR Studies of Peptide Derivatives |
title_sort |
circular dichroism and esr studies of peptide derivatives |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/78699853495394886028 |
work_keys_str_mv |
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