Flavonoids production by adventitious root culture of Belamcanda chinensis（L.）DC.

• Main Authors: Fang-Yi Chen, 陳芳儀
• Other Authors: Chin-Wen Ho
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/98882785834111759822

碩士 === 大同大學 === 生物工程學系(所) === 96 === The growth ratio of callus of Belamcanda chinensis reached to 9.3 times when cultured in MS liquid medium without growth regulators for 4 weeks. Callus regenerated into adventitious roots gradually during cultural period. Total flavonoid of methanolic extract in adventitious roots was 12.99±1.55 eq. iridin % DW. Five kinds of flavonoids (tectoridin , iridin , tectorigenin , isorhamnetin and irigenin ) in methanolic extract were analyzed by HPLC. Mature adventitious roots (> 3cm) were cultured in MS liquid medium supplemented with L-phenylalanine or yeast extract produced high isoflavonoid（3.8~4.1 % DW）. For scale up, adventitious roots were cultured in 1L bubble column bioreactor in 500 ml MS liquid medium for 4 weeks resulted 3.6-fold increase in biomass. The methanolic extracts in adventitious roots cultured in bubble column bioreactor exhibited 1.3-fold in total flavonoid and 1.2-fold in total isoflavonoid, compared to 3-year-old medicinal rhizome. Tectoridin was major isoflavonoid in medicinal rhizome but iridin accumulated in large amount in adventitious root by in vitro culture.