| Summary: | 博士 === 大同大學 === 生物工程學系(所) === 96 === Enterovirus infect the gastrointestinal tract .Taiwan is located in temperate climates, so that there were enterovirus cases increased circulation in summer and early fall since 1998. Due to the diversity of disease manifestations, diagnoses cannot be made on clinical grounds alone. However, recent developments in molecular detection technology make it probable that enterovirus diagnosis will increasingly be achieved by non-culture-based methods, particularly nucleic acid amplification methods such as PCR. In this study, we combined RT-nested-PCR and molecular epidemiological method to detect and identify total 200 suspected enterovirus cases that collected from hospital of Taipei city during 2005 April and 2005 October. The result showed that RT-PCR positive detection rate for enterovirus was 46.5% (93/200 ) and also confirmed true positive detection rate for enterovirus was 68 % (136/200) by Seminest- and Nest-PCR. There are two seasonal peaks appeared in April-June and October within Taipei area. According to the analysis of statistic method, it showed that there were no significant difference for enterovirus infectious rate between Sexual、Age and environmental conditions.
The major clinical syndrome for those enterovirus-positive patients are fever (86.8 %) 、vesicular lesions on mouth ( 83.8 % )、poor appetite (66.9%)、vesicular lesions on hands and foots (62.5%)、skin rash (52.9%)、cough (35.3%) and unusual sleepiness (27.2%). Total 136 clinical samples of Non-polio enterovirus from 2005 April to October were analyzed by sequence analysis and then identify serotype. The result showed that there are 11 serotype detected in this study. The distribution percentage of each serotype was CA16：52.0% (72/136)；EV71：11.0% (16/136)；CA6：9.0% (12/136)；ECV11：9.0% (12/136)；CA2：6.0% (8/136)；CB1：3.0% (4/136)；CA10：2.0% (3/136)；ECV6、ECV30、CA4 and CA5, 1% (1/136), unknown type,4.0%, respectively.
The molecular evolution result of 5’NCR sequence of total 16 clinical samples isolated from this study were clustered as three major subgroup (Subgroup A、B and D).There was only one sample (C-207) belonged to 1998 outbreak of enterovirus 71 in Taiwan ( Subgroup B).Subgroup A ( include 11 isolates of EV71) were more closely related to the epidemic strain that isolated from China. However it showed that Subgroup D, includes 4 clinical samples, were classified as one unique subgroup specific for Taiwan isolates.
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