The biological effects of curcumin and far infrared radiation in K562 and THP-1 cells

• Main Authors: Ming-Hsiang Hsieh, 謝明祥
• Other Authors: Wen-Tan Huang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/2jtr78

碩士 === 元培科技大學 === 影像醫學研究所 === 96 === The curcumin possess the ability to regulate cell signal transcription pathways (nuclear factor κ B; NFκB, Bax) inhibit cancer cells proliferation. It also involves the down-regulation of the activities of matrix metalloprotenases (MMPs) and inflammatory mediators. The FIR has inhibition effects on tumor cell growth and therapeutic functions in autoimmunity and inflammation diseases. This study evaluated the biological effects of both curcumin and far infrared radiation in K562 and THP-1 cells. In the curcumin and FIR anticancer study, we treated K562 cells with increased concentrations of curcumin (1 μM, 10 μM, 25 μM, 50 μM and 100 μM), after 24 hrs, the survival numbers of K562 cells decreased to 89%, 50% and 30% in response to treated with 1 μM, 10 μM and 25 μM of curcumin, compared with the vehicle control. However, when k562 cells were radiated with FIR 30 min then treated with the same concentrations of curcumin in the above experiments, data showed k562 cell numbers were decreased to 73%, 35% and 27%. In the curcumin and FIR anti-inflammatory study, we treated THP-1 cells with low concentrations of curcumin (1 μM, 5 μM and 10 μM) for 24 hour; we found here was no significant effect in activity of MMP-2. But when THP-1 cells pre-stimulated with 100 nM/ml PMA (phorbol-12-myristate-13-acetate, compound can bind to protein kinase C and induces the activity of MMP-2 and MMP-9) then treated with the same concentrations as above, zymography analysis showed that curcumin caused significant inhibition in the activity of MMP-2 and MMP-9 from 100% (control) to 90% (1 μM), 83% (5 μM) and 68% (10 μM). Therefore, curcumin decreased the activity of MMP-2 and MMP-9 in PMA- stimulated THP-1 cells in dose dependent manner. In addition, the study used different radiated time of far infrared (5 minute, 10 minute and 15 minute) to radiate the THP-1 cells which were pre-stimulated with 100 nM/ml PMA for up-regulation of MMP-2 and MMP-9 activities. The data demonstrate that far infrared suppresses PMA-induced expression of MMP-2, MMP-9 in a time-dependent manner in THP-1 cells. The NO plays an important role in numerous tissues correlate with inflammatory. Therefore, in the study, we assessed the effects of curcumin and far infrared on NO production in lipopolysaccharide (LPS) activated RAW264.7 (mouse macrophage cells). Results showed that curcumin could decrease NO production in LPS-stimulated RAW264.7 cells in a dose dependent effect. However, far infrared could not effectively decrease NO production in LPS-stimulated RAW264.7 cells. In NBT assay, the data demonstrated that curcumin at low concentrations could reduce the superoxide activity of PMA-stimulated THP-1 cells in a dose dependent manner. But, far infrared had no significant effect in reduction of superoxide activity. These above study results demonstrate that curcumin can decrease the survival rate of k562 cells line, inhibit the activities of MMPs, and reduce NO production and superoxide activity in stimulated macrophages. However, far infrared jut can inhibit the k562 cells line survival rate and the activities of MMPs. Thus, curcumin and far infrared can be a promising clinical therapy of anti-cancer and anti-inflammation.