Development of detection methods and environmental hydrology of Shigella spp. and enterohemorrhagic E.coli in environmental waters : the case of Yunlin and Chiayi

• Main Authors: Yu-jung Tseng, 曾鈺容
• Other Authors: Bing-Mu Hsu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/51487241972755638263

碩士 === 國立中正大學 === 地震研究所暨應用地球物理研究所 === 97 === Waterborne disease is the world''s public health issues of concern. The main purpose of this study is to develope the detection procedures of Shigella spp. and enteroinvasive Escherichia coli (EIEC) in water samples. Their pathogenic mechanism is similar and toxicity is located at the invasion plasmid antigen H (ipaH). In total of 93 sites of water samples were taken from 9 drinking water treatment plants and Puzih River located in Yun-Lin and Chia-yi counties. Comfirmative microbiological tests for Shigella spp. and EIEC include polymerase chain reaction (PCR) assay, biochemical test and serological test. In addition, we also investigated the relationship between bacteria and various water quality paramters. All the water samples were concentrated with membrane filtrateion method and enriched with selected media, SS and XLD, expressing colonies in 76 out of the 93 water samples positive (81.7%) with candidates of Shigella and EIEC. Five out of 93 samples positive (5.3%) with a Shigella-, and EIEC-specific polymerase chain reaction targeted the ipaH gene. Guided by the biochemical tests and serological test for 5 ipaH gene positive samples, all were identified as EIEC. Significant differences were not observed between the presence/absence of EIEC and turbidity, total coliforms. The presence of EIEC should be considered a potential health threat when associated with human activities in watershed.