Manufacturing and evaluating of periodontal regeneration membrane
碩士 === 長榮大學 === 生物科技學系(所) === 97 === The aim of this study is to investigate a novel non-biodegradable periodontal regeneration membrane. Evaluate the influence of the membrane on human osteoblast-like cells, MG-63, in vitro. According to the results, try to find the most appropriate membrane. In t...
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Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2009
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Online Access: | http://ndltd.ncl.edu.tw/handle/6cj92a |
Summary: | 碩士 === 長榮大學 === 生物科技學系(所) === 97 === The aim of this study is to investigate a novel non-biodegradable periodontal regeneration membrane. Evaluate the influence of the membrane on human osteoblast-like cells, MG-63, in vitro. According to the results, try to find the most appropriate membrane. In this study the commercial pure titanium (CP-Ti) was used as non-biodegradable membrane. To modify titanium surface via sulfuric acid treating (AT). In this study chitosan and genipin was used to prepare a novel and biodegradable periodontal regeneration membrane, genipin cross-linked chitosan/ tricalcium phosphate composite barrier (GCT). Characterize the modified titanium surface by FESEM and XRD. The results indicate the concentration of sulfuric acid larger than 30%, there is significantly effective on the membrane surface. After cell culture test, we found cells proliferation were significantly slow on roughness surface and high concentration GCT membrane. The results of MTT test revealed that titanium membranes and the GCT membranes with low concentration genipin are no cytotoxin to MG-63 cells. Mineralization staining and gene expression results showed that the surface roughness of AT40 could promote osteoblast differentiation. The results of this study provided that acid-etched titanium substrate and GCT membrane have potential application in guided tissue regeneration research.
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