| Summary: | 碩士 === 中國醫藥大學 === 醫學檢驗生物技術學系碩士班 === 97 === DNA methylation is an epigenetic modification characterized by the covalent addition of a methyl (CH3) group at 5-carbon position of the cytosine ring, and typically occurs at CpG dinucleotides. DNA methylation is a major epigenetic modification of the genome that regulates crucial aspect of its function. It is also thought to play an important role in tumorgenesis. Oral cancer is the fourth leading cancer in the male population in Taiwan. Furthermore, the number of death was increased every year. In many Asia cultures chewing areca is known to be a strong risk factor for developing oral cancer. Therefore, a 4-NQO plus arecoline induced animal model was used to confirm areca really increasing occurrence of oral cancer. To find more genes which are aberrant methylated by arecoline, CpG island microarrays were used to analyze aberrant methylation in animal oral cancer tissues. Our data showed that 42 genes are hypermethylation and 9 genes are hypomethylation in 4-NQO plus arecoline induced animal oral cancer tissues. Four specific genes have been confirmed by methylation-specific PCR assays. Taken together, DNA methylation level is associated with the areca-associated oral tumorigenesis. The mechanisms of specific hyper- or hypo-methylation genes involved in the modulation of oral cancer is under investigated.
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