| Summary: | 碩士 === 中國醫藥大學 === 基礎醫學研究所 === 97 === The metabolic syndrome is a major risk factor and it raised the mortality in recently years. This study focuses on the hypertension and diabetes. Both these two diseases caused the heart failure in the end. In the reviews, cardiac apoptosis was found in hypertension and diabetes, but very limited information regarding the effects of exercise training on cardiac apoptosis in hypertension and diabetes was available. The purpose of this study was to evaluate the therapeutic effects of exercise training on hypertension and diabetes related cardiac apoptosis. Spontaneously hypertensive rats and streptozotocin-induced diabetic rats were divided into sedentary group (SHR and DM) and unwanted running exercise on treadmill 1 hour daily, 5 sections per week, for 12 and 8 weeks (SHR-EX and DM-EX). Wistar Kyoto rats (WKY) and Wistar rats (Control) as a negative control. After exercise training or sedentary status, the excised hearts were measured by H&E stain, TUNEL assay and western blotting. Protein levels of apoptotic pathway, including death and mitochondria dependent pathways, were increased in both SHR and DM groups; and survival related proteins were decreased in both SHR and DM groups compared with normal groups (WKY and Control). Cardiac mitochondria and death receptor dependent apoptotic pathway were decreased and survival pathway was increased in both SHR-EX and DM-EX groups compared with normal groups (WKY and Control). Exercise training might suppress cardiac apoptotic pathways and elevated cardiac survival pathway in hypertensive and diabetic rat models. The findings may provide one of the possible therapeutic approaches for preventing cardiac apoptosis in hypertension and diabetes.
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