| Summary: | 碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 97 === Abstract
Many studies indicated that plasma high levels of homocysteine(Hcy) might enhance occurrence of neuronal injury or death, and it could be also one of the high risk factor in cardiovascular disease. Workers in high temperature and humidity were easy to induce heatstroke formation. Studies found that the heatstroke patients and animals all displayed the central ischemia, hypoxia, neuronal damage, and high mortality. Many literatures suggested that the patients of cardiovascular diseases might be easier to evoke heatstroke induction. However, there is less attention to investigate what role of the Hcy played during heatstroke induction, and whether plasma high levels of Hcy could be also one of high risk factor in heatstroke induction. So far, there is no one strategy can be to tall therapy the heatstroke- induced damage. Consequently, it seems more important to prevent the heatstroke induction. The main purpose of this study is to observe the effects of Hcy on heatstroke induction and heatstroke- induced damage by a rat model. Rats under anedthesia were exposed to an ambient temperature(42℃) to induced heatstroke. We observe effects of continuous infusion with high concentration Hcy(17.5 �慊/min, i.v.) or 0.9％ normal saline(50 �愮/min, i.v.) on heatstroke. Our results showed that rats with continuous infusion high concentration Hcy were significantly shrunk the duration time of heatstroke induction, but raised the beats of heart rate and colonic temperature. “The situations of heatstroke- induced cerebral ischemia, hypoxia and neuronal damage” were apparently revealed ahead of time in rats with continuous infusion Hcy group. During heatstroke induction, we also observe the effects of continuous infusion high concentration Hcy on levels of free radicals and cytokines in rats. The similar results were also obtained, the plasma levels of free radicals and cytokines were significantly expressed in advance in rats of high concentration Hcy group. Meanwhile, the DNA damage of tissue was also obtained ahead of time high concentration Hcy during heatstroke. According to our results, we speculated that animals with high levels of Hcy may probably be one of high risk factors to early induce heatstroke formation, and beforehand reveal or aggravate the heatstroke- induced damage.
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