| Summary: | 碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 97 === Ludwigia octovalvis (Jacq.) P. H. Raven (Onagraceae) is an aquatic plant widely distributed in the wet area of Taiwan. It is used as a traditional treatment for edema, nephritis, and hypertension. The results showed that ethanol extract of L. octovalvis (EELO) possessed the lowest IC50 value against the 3T3-L1 cells. Fraction 3 was found to induce significant cell death in 3T3-L1 cells. We investigated the effect of chlorophyll a (CHL-a) isolated from the leaves of L. octovalvis on the apoptotic pathway in 3T3-L1 cells. The results showed that CHL-a was the most effective with an IC50 value at 48 h. At concentration of 5~30 nM, CHL-a exhibited a dose-dependent accumulation of the Sub-G1 peak and caused G0/G1-phase arrest. After 48 h treatment, 30 nM CHL-a reduced the cell viability, induced the appearance of DNA fragments, and increased the activity of the caspase-3. Western blot data revealed that CHL-a decreased level of Bcl-2, and increased the expression of Bax and CD95 (APO-1/CD95) in a time- and a dose-dependent manner. Furthermore, CHL-a exposure up-regulated the levels of AMPK and P-AMPK, but the expression of PPAR-γ was down-regulated. These data indicate that CHL-a induced apoptosis of mature adipocytes through mediating the CD95 (APO-1/CD95) system, and modulation of Bcl-2 family proteins and caspase-3 activity, as well as exerting anti-adipogenesis ability by activating the AMPK signaling pathway.
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