The study of cystamine on the apoptosis and its related mechanisms on heart of NZB/W F1 mice

• Main Authors: Sin-Lun, 李欣倫
• Other Authors: Bor-Show Tzang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/82870020760297447923

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 97 === Systemic lupus erythematosusb is an autoimmune disease, stuies indicate that SLE can cause serious organ damage, Especially suffering from SLE may cause which is the cause of decline of cardiac fuction. This is the main factor in heart disease. Apotosis in the course of the disease plays an important role, Studies point out apoptosis is related to TG2, at the same time TG2 may be associated with SLE , and cystamine is the TG2 inhibitor. Therefore the main discuss is through use of cystamine about the relation between SLE and heart .The 28-week-old BALB/c and NZB/W F1 mice are injected with cystamine for 14day in a row, we analysis BALB/c and NZB/W F1 mice physical and pathology,we use 2-D electrophoresis analysis,we found cystamine reduce CKMB protein performance and improvement myocardial cell damage , we investigate the myocardial cell apoptosis, survival and hypertrophy, Based on physiology data the cystamine can effectively prevent NZB/W F1 mice from hypertrophy,and we also detect with use of Western Blot analysis and 2-D electrophoresis analysis , cystamine can reduse cardiac hypertrophy performance of FABPand ANP protein, induce hypertrophy pathway,IL6 MEK5-ERK5 and p-p38, c-jun as well. In TUNEL assay it can be observed that cystamine can reduce myocardial cell apoptosis . At the same time Western Blot analysis to prove cystamine can reduce cardiac cell apoptosis and Deth receptor and Mitochondria through theses two pathsway, NF-κB protein expression significantly increased furthermore, it promots cell survival, which can reduce cardiac hypertrophy,apoptosis.