| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 97 === Solanum nigrum L., which belongs to the Solanaceae family, has been used traditionally to treat various ailment such as pain, inflammation and fever. It is believed to have anti-oxidative, anti-inflammatory, hepatoprotection. The purpose of this study were to investigate the inhibitory effects of water extract of Solanum nigrum L. (SNWE) and polyphenolic extract of Solanum nigrum L. (SNPE) on the angiogenesis and metastasis in B16-F1 cells. First, SNWE and SNPE exhibited an inhibitory effect on the migration ability by wound healing assay and Boyden chamber assay. Both SNWE and SNPE showed to reduce MMP-2 and MMP-9 in B16-F1 cells. The results of the Western blotting assay demonstrated that the expression levels of Ras, PI3K, phospho-Akt, and NF-κB in the SNWE and SNPE treated B16-F1 cells were reduced ; the expression levels of PKCα, PKCδ, phospho-JNK, phospho-p38 were also decreased, and such as phospho-ERK was unaffected. In angiogenesis experiment, SNWE and SNPE exhibited an inhibitory effect on the angiogenesis by chorioallantonic membrane inoculation and plug assay. Further investigation revealed that the anti-metastatic effect of SNWE was also evident in a C57Bl/6 mice model. The effects of dietary administration of SNWE on metastasis induced by B16-F1 cell in C57BL/6 mice. Accompanied with treated with SNWE at high concentrations indicated mice viability better. Taken together, the findings proved the inhibitory effect of SNWE and SNPE on the angiogenesis and metastasis of melanoma cells in vitro and in vivo.
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