Matrix metalloproteinase-12 increase and elastin degradation in astroglia infected with Toxoplasma gondii

• Main Authors: Cheng-You, 盧正祐
• Other Authors: 蔡崇弘
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/09002510527526774908

碩士 === 中山醫學大學 === 醫學研究所 === 97 === Toxoplasma gondii is an obligate intracellular protozoa in the mammal animals, including human. The host infected with T. gondii wich can induce toxoplasmic encephalitis. In this study, astroglia infected with TS-4 strain T. gondii tachyzoite and to investigate the changes of matrix metalloproteinase-12 (MMP-12) and its substrate elastin in the pathogenesis. The results showed that MMP-12 was found in the cell clutered medium of T. gondii infected-astroglia and significantly increased at 24hrs and 48 hrs post-infection (PI). Similarly, the expression of elastin was also increased at the same time points. The inhibitory test of astroglia infected with T. gondii was co-cultured with four different concentrations of MMPs inhibitor GM6001 (0.01mM, 0.1mM, 0.5mM and 1mM). The concentrations of GM6001 on 0.5mM and 1mM were significantly reduced the astroglia degradation, and the expression of MMP-12 and elastin in astroglia infected with T. gondii. These results suggested that MMP-12 may contribute to elastin degradation occurring in astroglia infected with T. gondii.