Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana

• Main Authors: Fu-Sen Hsu, 許富森
• Other Authors: H-K Yak
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/56377236742591490469

碩士 === 中原大學 === 化學研究所 === 97 === According to the statistical analysis of Department of Health in Taiwan, the malignant tumor (cancer) has been the highest in the ten leading causes of death in the past 26 years and the death toll continued to rise to greater than 40,000 people in 2007. Consequently, anti-cancer drug market and the demand have also gradually been increasing accordingly. One research in this area is to find more environmental friendly methods or green manufacturing process for the production of anti-cancer drug. This experiment uses Taiwan's native yew Taxus sumatrana as the for the extraction of taxol and 10-deacetyl baccatin III (10-DAB) by pressurized water extraction (PWE). High performance liquid chromatography (HPLC) equipped with a carbon-18 column was used for the quantitative determination of the extracts. The results of PWE were compared to conventional organic solvent extraction. Experimental conditions including leaves crushing, pH of extraction, extraction pressure, mixing of the sample with sand, and static extraction time were studied. Crushing of the leaves and static extraction time were found to be the more influential parameters. For taxol, our results showed that 31 mg taxol can be obtained from one kg of dry leaves, while 1698 mg 10 - deacetylation baccatin III can be obtained from one kg of dry leaves.