Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana
碩士 === 中原大學 === 化學研究所 === 97 === According to the statistical analysis of Department of Health in Taiwan, the malignant tumor (cancer) has been the highest in the ten leading causes of death in the past 26 years and the death toll continued to rise to greater than 40,000 people in 2007. Consequentl...
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ndltd-TW-097CYCU50650362015-10-13T12:04:54Z http://ndltd.ncl.edu.tw/handle/56377236742591490469 Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana 利用高壓水萃取紅豆杉葉中紫杉醇及10-去乙醯巴卡亭III之研究 Fu-Sen Hsu 許富森 碩士 中原大學 化學研究所 97 According to the statistical analysis of Department of Health in Taiwan, the malignant tumor (cancer) has been the highest in the ten leading causes of death in the past 26 years and the death toll continued to rise to greater than 40,000 people in 2007. Consequently, anti-cancer drug market and the demand have also gradually been increasing accordingly. One research in this area is to find more environmental friendly methods or green manufacturing process for the production of anti-cancer drug. This experiment uses Taiwan's native yew Taxus sumatrana as the for the extraction of taxol and 10-deacetyl baccatin III (10-DAB) by pressurized water extraction (PWE). High performance liquid chromatography (HPLC) equipped with a carbon-18 column was used for the quantitative determination of the extracts. The results of PWE were compared to conventional organic solvent extraction. Experimental conditions including leaves crushing, pH of extraction, extraction pressure, mixing of the sample with sand, and static extraction time were studied. Crushing of the leaves and static extraction time were found to be the more influential parameters. For taxol, our results showed that 31 mg taxol can be obtained from one kg of dry leaves, while 1698 mg 10 - deacetylation baccatin III can be obtained from one kg of dry leaves. H-K Yak 葉華光 2009 學位論文 ; thesis 98 zh-TW |
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碩士 === 中原大學 === 化學研究所 === 97 === According to the statistical analysis of Department of Health in Taiwan, the malignant tumor (cancer) has been the highest in the ten leading causes of death in the past 26 years and the death toll continued to rise to greater than 40,000 people in 2007. Consequently, anti-cancer drug market and the demand have also gradually been increasing accordingly. One research in this area is to find more environmental friendly methods or green manufacturing process for the production of anti-cancer drug.
This experiment uses Taiwan's native yew Taxus sumatrana as the for the extraction of taxol and 10-deacetyl baccatin III (10-DAB) by pressurized water extraction (PWE). High performance liquid chromatography (HPLC) equipped with a carbon-18 column was used for the quantitative determination of the extracts. The results of PWE were compared to conventional organic solvent extraction. Experimental conditions including leaves crushing, pH of extraction, extraction pressure, mixing of the sample with sand, and static extraction time were studied. Crushing of the leaves and static extraction time were found to be the more influential parameters. For taxol, our results showed that 31 mg taxol can be obtained from one kg of dry leaves, while 1698 mg 10 - deacetylation baccatin III can be obtained from one kg of dry leaves.
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author2 |
H-K Yak |
author_facet |
H-K Yak Fu-Sen Hsu 許富森 |
author |
Fu-Sen Hsu 許富森 |
spellingShingle |
Fu-Sen Hsu 許富森 Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana |
author_sort |
Fu-Sen Hsu |
title |
Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana |
title_short |
Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana |
title_full |
Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana |
title_fullStr |
Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana |
title_full_unstemmed |
Pressurized Water Extraction of Taxol and 10-deacetyl baccatin III from Needles of Taxus sumatrana |
title_sort |
pressurized water extraction of taxol and 10-deacetyl baccatin iii from needles of taxus sumatrana |
publishDate |
2009 |
url |
http://ndltd.ncl.edu.tw/handle/56377236742591490469 |
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