Molecular studies on activation-tagged Antrodia cinnamomea and analysis of its active compounds

• Main Authors: Chien-fu Chen, 陳建甫
• Other Authors: Chin-fun Chen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/69838063602870764765

碩士 === 朝陽科技大學 === 生物技術研究所 === 97 === Antrodia cinnamomea is well known in Taiwan as a source of traditional Chinese medicine for the treatment of cancer and inflammation. In order to increase the content of active compounds, such as polysaccharides and triterpenoids in mycelia, Agrobacterium tumefaciens strain EHA105 harboring a standard binary vector pTAG-8 was used to generate the transgenic mycelia population. Sixteen transgenic mycelia (A~P) were obtained and confirmed by Southern blots(81.25％ of them with single copy insertion). Changes in mycelia color and growth rate were observed in all transgenic lines. Exopoly- ssacharides contents in transgenic lines A, C, K, L, N and O were significantly increased. Triterpenoids like dehydrosulphurenic acid (used for prostate cancer therapy) and dehydroeburicoic acid (used for anti-inflammation) in both fruiting bodies and mycelia were remarkably increased in transgenic lines D, H, I, J, K and A, B, D, H, I, respectively. Transgenic line H, containing high dehydrosulphurenic acid and dehydroeburicoic acid, was observed to have high scavenging activity. In transgenic line H, the DIMETHYLALLYL TRYPTOPHAN SYNTHASE(DTS) was as confirmed by gene expression. Mutation in DTS might cause accumulation of dehydrosulphurenic acid and dehydroeburicoic acid in transgenic line H. Key words: Antrodia cinnamomea, Agrobacterium tumefaciens, Activation tagging.