Studies on the antifungal substance, zwittermicin A, from local Bacillus thuringiensis isotate (D1-10a)
碩士 === 朝陽科技大學 === 生物技術研究所 === 97 === Using polymerase chain reaction (PCR) with two pairs of oligonucleotide primers to detect both ZmaR and Zwit genes. In this study, we were able to efficiently screen the local Bacillus thuringiensis (B.t) isolates that produced zwittermicin A. Of the 25 B.t isola...
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2009
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| Online Access: | http://ndltd.ncl.edu.tw/handle/19401203204004269321 |
| Summary: | 碩士 === 朝陽科技大學 === 生物技術研究所 === 97 === Using polymerase chain reaction (PCR) with two pairs of
oligonucleotide primers to detect both ZmaR and Zwit genes. In this study,
we were able to efficiently screen the local Bacillus thuringiensis (B.t)
isolates that produced zwittermicin A. Of the 25 B.t isolates tested, B.t
D1-10a exhibited the highest antifungal activity which can inhibit the
growth of Micrococcus luteus、Fusarium solani、Phytophthora capsici、
Phytophthora Citricola、Pythium myriotylum.Antifungal activity can be
enhanced by the addition of ferric ion to trypticase soy broth. And the
cation-exchange hromatography (Amberliter IRC-50) purified supernatant
of broth still showed inhibition against the mycelial growth (from
zoospores) of Pythium myriotylum.The antibiotic substances were further
purified by reverse phase C18 HPLC. A single peak was eluted at 6.5 to
7.5 minutes from HPLC. The antibiotic substances from HPLC were
identified by LC-TOF MS. The molecular weight of this compound was
determined to be approximately 387.1 Da, that was similar to the
confirmed zwittermicin A producted by B.cereus UW85. In this study, the
purified zwittermicin A-like compound can also inhibit the germination of
P. myriotylum zoospores.
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