Identification of diabete-related plasma and liver protein markers by using post-translational proteomic approaches and a proteomic approach to decipher the growth inhibition and killing mechanism of nano-gold particles on human leukemia cells

• Main Authors: CHAO, YA-LI, 趙亞麗
• Other Authors: 陳翰民
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/09122331437848612324

碩士 === 輔仁大學 === 生命科學系碩士班 === 97 === In this study, we utilized proteomic approaches to investigate the differentially expressed plasma protein related to diabetes. The streptozotocin (STZ) diabetic rats were utilized for investigation. Using high fat diet to induce the diabete-like phenotype, the STZ treated and the control rat were examined for the blood sugar level and regrouped for proteomic analysis. By employing our developed affinity chromatography, the total proteome as well as the phosphoproteome and glycoproteome from the rat plasma samples were purified and examined. Briefly, albumin, vitamin D-binding protein precursor, and transthyretin were down- regulated, phosphorylated- complement inhibitory factor H, serum albumin, and alpha-2-HS-glycoprotein were up- regulated, and glycosylated –fibrinogen B beta chain, carboxylesterase precursor, serine peptidase inhibitor were up-regulated in the plasma of diabeteic rats. The physiological significance of the identified proteins with diabetes was discussed. On the other hand, proteomic techniques were also utilized to inspect the mechanism of death elicited by Nanogold particles, which was found effectively inducing apoptosis in human chronic leukemia cells (K562). In conjunction with proteomic techniques and systems biology analysis, we suggest that endoplasmic reticulum (ER) stress response may be the major cellular event elicited by Nanogold particles and induce cell death if unmanageable.