Production of bioactive peptides with hypotriglyceridemic action in seeds of transgenic soybean and Arabidopsis

• Main Authors: Erh-yun Hsu, 許薾勻
• Other Authors: Pei-Fang Lee
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/31811388876536124859

碩士 === 輔英科技大學 === 生物技術系碩士班 === 97 === Soybean is one of the most important crops for food, feed, and oil industrial applications. Val-Val-Tyr-Pro (VVYP) is a bioactive peptide with hypotriglyceridemic activity decreasing serum triglyceride level effectively. In this study, we intended to develop the transgenic plants as bioreactor for production of hypotriglyceridemic peptides. We generated transgenic soybean and Arabidopsis lines that expressed modified forms of the Gy1 and Gy5 of soybean seed storage protein glycinin containing tandem repeats of the bioactive peptide (VVYP). Segregation analyses of independently transformed Arabidopsis lines were performed by PCR and monitored by immunoblottings. These results indicated that the foreign genes were integrated with the genome of transformed Arabidopsis, and expressed modified glycinin in mature seeds. For improving the efficiency of Agrobacterium-mediated transformation of soybean (Glycine max L. cv. Kaohsiung sel. 10）, we established the transformation system by using embryonic tips as explants, adding SL-77 as well as carborundum in co-culture medium and wounding explants by sonication. The resistant transgenic soybeans were performed by GUS histochemical staining and showed GUS gene expression. These result demonstrated the feasibility of production of bioactive peptides with hypotriglyceridemic action in transgenic plants. In conclusion, the goal of this study expected to establish the platform for high value-added soybeans and develop the health food with hypotriglyceridemic action.