Down-regulation of RhoGDIα expression by TCF-4 and beta-catenin in hepatocellular carcinoma

• Main Authors: Min-Chiao Liao, 廖敏喬
• Other Authors: Der-An Tsao
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/25414296876790569799

碩士 === 輔英科技大學 === 醫事技術系碩士班 === 97 === In Taiwan, hepatocellular carcinoma ranks as the first and the second leading causes of death for male and female cancer patients, respectively. Recently, inappropriate activation of Wnt signaling pathway has been implicated in the development of HCC. Therefore, this study focused on searching for TCF-4 and beta-catenin regulated proteins. TCF-4 and β-catenin were transfected in Huh7. Our study found that TCF-4 and β-catenin down-regulated RhoGDIα expression in HCC by proteomic method. Furthermore, the levels of RhoGDIα expression in HCC tissue and peritumorous non-neoplastic liver tissue were determined by RT-PCR. The results indicated that RhoGDIα expression is down-regulated in human HCC compared with peritumorous non-neoplastic liver tissues. The functional analysis of RhoGDIα in tumor cell was defined further by apoptosis assay, MTT assay, migration and invasion assay. Ectopic expression of RhoGDIα altered tumor cell survival, apoptosis and migration. The results suggest that down-regulation of RhoGDIα expression plays a significant role in the progress of hepatocellular carcinoma.