| Summary: | 碩士 === 中華醫事科技大學 === 生物科技研究所 === 96 === Colon cancer is the major cause of malignancy-related deaths worldwide, and the most prevalent cancers in Taiwan. However, the genetic factors influencing its appearance remain far from being fully characterized. The chemokine（CXCL12），also known as stromal cell-derived factor-1 (SDF-1)，is a small protein that regulates leukocyte trafficking and it is variably expressed in a number of normal and tumor tissues. CXCL12 plays a key role in hematopoiesis, and it is involved in migration, homing, and survival of hematopoietic progenitors . CXCL12 is identified as ligand and its receptor CXCR4 have been implicated in colorectal cancer progression , including angiogenesis and metastasis. CXCL12-G801A , a single nucleotide polymorphism（SNP） in the 3' untranslated region, had been previously reported to correlate with breast and lung cancer in Iran. In this study we compared to colorectal cancer patients from a Taiwan series. The results from an independent analysis of CXCL12-G801A in colorectal cancer series of Taiwan origin in order to analyse the robustness of this association within a same population. The polymorphism was analysed with PCR and DHPLC（denaturing high performance liquid chromatography ）methods. There was significant difference in SNP distribution between colorectal cancer patients (n=40) and controls (n=20), P=0.0014. Furthermore, the analysis of allelic frequencies in both groups revealed that the frequency of G allele in patients and controls were 153 (78.9%) and 178 (89.9%), respectively. While the frequency of A allele in patients and controls were 41 (21.1%) and 20 (10.1%), respectively. Therefore it was revealed that patients had more A allele than controls. These differences were statistically significant (P=0.0026). Based on the above observation CXCL12-G801A can be considered as genotypes associated with colorectal cancer in Taiwan patients.
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