Molecularly Imprinted Electrochemical Sensor able to Enantioselectively Recognize L and D-tryptophan

• Main Authors: Ching-shiang Wang, 王慶翔
• Other Authors: Tzong-rong Ling
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/17683135005374292913

碩士 === 義守大學 === 生物技術與化學工程研究所碩士班 === 97 === In this research, trytophan sensing electrodes were prepared by molecular imprinting technique through electropolymerization process. The entro-selectivity of the electrode was investigated by using electrochemical method. And behaviors of the electro-active molecule were analyzed by CV method. The results revealed that strong peak of anodic current of trytophan appeared between 0.8 V to 1.0 V(vs. Ag/AgCl) from its π electron transfer. The interactions of related electro-active molecules were compared in the text. Furthermore, conducting polymer, polypyrrole, was used to prepared L and D trytophan imprinted electrode. Under some pretreatment condition, the target molecule was extracted from the imprinted electrode in NaOH solution. Then, its rebinding was induced by positive potential and quantitative adsorption was evaluated by coulomb method. The selectivity of the D and L- trytophan imprinted electrode was estimated respectively as: L/D=17.8±2.4, D/L=15.1±1.2 under the conditions: potential range -0.3 V~+1.2 V(vs. Ag/AgCl), scan rate: 0.1 V/sec, in 10 mM sample solution. This study will discuss the recognition ability affected by many factors including: pretreatment condition, extraction method of target molecules, concentration of target molecules, electropolymerization time, scanning rate, etc.