CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1
碩士 === 高雄醫學大學 === 生物化學研究所 === 97 === 英文摘要 Cisplatin (cis-diaminedichloroplatinum;CDDP), a platinum- based anticancer drug, is one of the most effective chemo- therapeutic agents. Its configuration includes two amines, two chlorines, and the center of platinum. The cytotoxic effect of cisplatin is ma...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2009
|
Online Access: | http://ndltd.ncl.edu.tw/handle/15514282042312948774 |
id |
ndltd-TW-097KMC05107006 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-097KMC051070062015-11-13T04:09:13Z http://ndltd.ncl.edu.tw/handle/15514282042312948774 CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 探討CEBPD調控SOD1基因表現參與在人類泌尿上皮癌細胞的cisplatin抗藥作用 Yan-Liang Lai 賴彥良 碩士 高雄醫學大學 生物化學研究所 97 英文摘要 Cisplatin (cis-diaminedichloroplatinum;CDDP), a platinum- based anticancer drug, is one of the most effective chemo- therapeutic agents. Its configuration includes two amines, two chlorines, and the center of platinum. The cytotoxic effect of cisplatin is mainly mediated by DNA damage, primarily forming intrastrand crosslinking DNA adducts and ROS production. However, its clinical efficacy is largely limited to its toxic effects on normal cells and the development of drug resistance within tumor cells. Therefore, understanding the molecular and cellular events upon cisplatin treatment will improve the development of therapeutic options for the patients. CCAAT/enhancer-binding protein delta (CEBPD) belongs to C/EBP transcription factor family. We previously found CEBPD mRNA and protein were induced by cisplatin in human urothelial carcinoma cell line NTUB1. In order to explore the biological roles of CEBPD in cisplatin response and resistance, various CEBPD- expressing stable clones in NTUB1 were established: N-V (empty vector pcDNA3); N-D#1 (WT, CEBPD full length); N-△DBD (Mutant, C-terminal basic leucine zipper domain of CEBPD 197-269 amino acids was deleted); N-R198A (Mutant, CEBPD amino acid198 Arginine was replaced by Alanine) and N-hd-pSi (CEBPD siRNA knocking down). The results showed that C-terminal DNA binding domain activity of CEBPD is essential for the cisplatin response and resistance. Furthermore, SOD1 was identified as a direct target of CEBPD and contributed to CEBPD-mediated cisplatin resistance in NTUB1 cells. Thus, CEBPD might be a potential target for clinical treatment of cisplatin resistant bladder cancers. The underlying molecular mechanism of CEBPD-mediated SOD1 expression in cisplatin response/resistance needs to be further explored. A-Mei Huang 黃阿梅 2009 學位論文 ; thesis 94 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 高雄醫學大學 === 生物化學研究所 === 97 === 英文摘要
Cisplatin (cis-diaminedichloroplatinum;CDDP), a platinum- based anticancer drug, is one of the most effective chemo- therapeutic agents. Its configuration includes two amines, two chlorines, and the center of platinum. The cytotoxic effect of cisplatin is mainly mediated by DNA damage, primarily forming intrastrand crosslinking DNA adducts and ROS production. However, its clinical efficacy is largely limited to its toxic effects on normal cells and the development of drug resistance within tumor cells. Therefore, understanding the molecular and cellular events upon cisplatin treatment will improve the development of therapeutic options for the patients. CCAAT/enhancer-binding protein delta (CEBPD) belongs to C/EBP transcription factor family. We previously found CEBPD mRNA and protein were induced by cisplatin in human urothelial carcinoma cell line NTUB1. In order to explore the biological roles of CEBPD in cisplatin response and resistance, various CEBPD- expressing stable clones in NTUB1 were established: N-V (empty vector pcDNA3); N-D#1 (WT, CEBPD full length); N-△DBD (Mutant, C-terminal basic leucine zipper domain of CEBPD 197-269 amino acids was deleted); N-R198A (Mutant, CEBPD amino acid198 Arginine was replaced by Alanine) and N-hd-pSi (CEBPD siRNA knocking down). The results showed that C-terminal DNA binding domain activity of CEBPD is essential for the cisplatin response and resistance. Furthermore, SOD1 was identified as a direct target of CEBPD and contributed to CEBPD-mediated cisplatin resistance in NTUB1 cells. Thus, CEBPD might be a potential target for clinical treatment of cisplatin resistant bladder cancers. The underlying molecular mechanism of CEBPD-mediated SOD1 expression in cisplatin response/resistance needs to be further explored.
|
author2 |
A-Mei Huang |
author_facet |
A-Mei Huang Yan-Liang Lai 賴彥良 |
author |
Yan-Liang Lai 賴彥良 |
spellingShingle |
Yan-Liang Lai 賴彥良 CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 |
author_sort |
Yan-Liang Lai |
title |
CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 |
title_short |
CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 |
title_full |
CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 |
title_fullStr |
CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 |
title_full_unstemmed |
CEBPD-induced SOD1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line NTUB1 |
title_sort |
cebpd-induced sod1 expression mediates cisplatin resistance in human bladder urothelial carcinoma cell line ntub1 |
publishDate |
2009 |
url |
http://ndltd.ncl.edu.tw/handle/15514282042312948774 |
work_keys_str_mv |
AT yanlianglai cebpdinducedsod1expressionmediatescisplatinresistanceinhumanbladderurothelialcarcinomacelllinentub1 AT làiyànliáng cebpdinducedsod1expressionmediatescisplatinresistanceinhumanbladderurothelialcarcinomacelllinentub1 AT yanlianglai tàntǎocebpddiàokòngsod1jīyīnbiǎoxiàncānyǔzàirénlèimìniàoshàngpíáixìbāodecisplatinkàngyàozuòyòng AT làiyànliáng tàntǎocebpddiàokòngsod1jīyīnbiǎoxiàncānyǔzàirénlèimìniàoshàngpíáixìbāodecisplatinkàngyàozuòyòng |
_version_ |
1718129144873615360 |