Enantiomeric separation of dl-methylphenidate in human plasma by capillary electrophoresis

• Main Authors: Shih-Chieh Lee, 李詩潔
• Other Authors: Shou-Mei Wu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/52784021133325766777

碩士 === 高雄醫學大學 === 藥學研究所 === 97 === A simple and rapid capillary electrophoresis (CE) method was established for the chiral determination of dl-methylphenidate (dl-MP) in human plasma. This CE method was applied to analyze dl-MP to evaluate clinical efficiency. After liquid-liquid extraction with n-hexane, the sample was loaded by electrokinetic injection (6 kV, 200 s). The stacking step and separation were performed at 20 kV and detected at 200 nm using phosphate buffer (50 mM, pH 3.0) containing 30 mM triethanolamine and 20 mM hydroxypropyl-β-cyclodextrin. During method validation, calibration curves were linear (r≧ 0.998) over a range of 1 – 80 ng/ml. For the intra-day and inter-day precision and accuracy evaluation, the RSD and RE were all less than 5.5%. The limit of detection was 600 pg/ml(S/N=4, sampling 200 s at 6 kV). Our method had been further applied to investigate the pharmacokinetic study of four healthy volunteers by dosing dl-MP tablets according to their body weights (0.3 mg/kg). The results were coincident with reference data. Advanced applications in clinic are under investigation, we expect this study could provide more information for dl-MP administration.