The Study of Effects of Pharmaceuticals for Blood Coagulation and Anti-Blood Coagulation by Optical Tweezers System

• Main Authors: Kui-Teng Huang, 黃葵騰
• Other Authors: Bor-Wen Yang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/15131273434993489701

碩士 === 明新科技大學 === 電機工程研究所 === 97 === Blood is the most important medium to transport living matters in human body. As wounds appear outside or inside one’s body, the speed of blood coagulation is critical to maintain one’s life. Capable of non-contactive interaction with live cells, optical tweezers become powerful tools for micro-manipulation and are commonly used in pN-range biological kinetics. Single-beam optical tweezers were established in this work to study the coagulation between blood cells and the effects of pharmaceuticals for blood and anti-blood coagulation. A tunable IR laser of 1064 nm and 300 mW, polarization optics, modification optics and an inverted biological microscope with 100× oil lens were employed to configure the optical tweezers system. The X-Y stage was motorized to induce viscosity, by which the interaction of the tweezers on the particle was determined. By a sample bead of ~3 μm, the relation between the laser power and the derived interaction of the tweezers on any focused particle was established as database. Gradually reducing the power of the spot on a trapped blood cell to a critical value, the interaction of the spot on the cell was balanced with the coagulation by the surrounding blood cells. At this moment, the trapped cell was released and then attracted to the coagulated blood clot, when the trapping force of the tweezers, and thus the coagulating force between the blood cells, could both be determined. In the study, we not only observed the process of blood coagulation by the tweezers, but verified the effects of pharmaceuticals on the efficiency of blood coagulation. Without medicine, the force of coagulation between usual blood cells was found around 2.0 pN; with heparin, the coagulating force attenuated to about 1.4 pN; while treated with Tranexamic Acid, it was raised to 2.5 pN. With confirmed anti-coagulation medicine, the life of the patients with cerebral apoplexy or cardiovascular embolism may be well extended; with verified hemagglutination medicine, the first-aid services on the sick with hemophilia or internal hemorrhage will be advanced as well.