Determination of methylmercury in liver of rats by headspace solid phase microextraction combined with gas chromatography-mass spectrometry

• Main Authors: Kuei-Chen Chang, 張桂禎
• Other Authors: 李茂榮
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/71196232443076770541

碩士 === 國立中興大學 === 化學系所 === 97 === Mercury exists in environment in different forms. The more toxic form, organic mercury, can be converted from elemental and inorganic mercury by biological process. Methylmercury which can be bioaccumulated through the food chain is one of the most toxic organic mercury. Methylmercury can arise from dysfunctions of the central nervous system in human and be accumulated in liver, kidney and brain easily. Headspace solid-phase microextraction combined with gas chromatophy-tendem mass spectrometry ( headspace SPME-GC-MS-MS ), was developed to determine trace methylmercury in liver of mice in this study. The linear range of the method was 0.1-100 μg/g with correlation coefficient at 0.9981. The limit of detection was 1.4 ng/g. The precisions were 9.1 % and 6.3 % when the concentrations were 1 μg/g and 50 μg/g, respectively. The recovery of the method was 97 %. The method was simple, rapid, solvent free, reliable and sensitive. The proposed method was tested by analyzing real liver samples of mice for methylmercury residues. Results of this study demonstrate the accuracy of the headspace SPME-GC-MS-MS method for analyzing methylmercury in liver samples.