The development of multiple analytical techniques coupled with disposable screen-printed electrodes

• Main Authors: Mei-Hsin Chiu, 邱梅欣
• Other Authors: 曾志明
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/30191463538923977980

博士 === 國立中興大學 === 化學系所 === 97 === Nowadays electroanalysis combined with other analytical work systems are well known for its broad application. In this doctoral thesis work, variety of electrochemical detectors developed with simple, low cost, easy useable screen-printed carbon electrodes and then applied for a spectrum of analysis. The first two chapters explain the fabrication of different types of electrodes and brief introduction of techniques used in this research work. Section I of Chapter III explains the use of screen-printed carbon electrode, SPCE as a working electrode in the development of an electrochemical detector for the assay of aminoglycoside antibiotics. Determination of oxalic acid by the combination of electrochemiluminescence and electroanalysis techniques are also studied with simultaneous analysis of current and light intensity response and this technique not only effective in increasing the order of magnitude but also good for complex quantitative analysis with efficacy. While in Section II, SPCE working electrode combined with amperometric flow injection analysis used for analysis of penicillin type antibiotics; amoxicillin with MnO2 reactor. It is based on the well-known chemical conversion of catechols to o-quinones by MnO2 that can be subsequently easily determined by electrochemical reduction at inexpensive screen-printed carbon electrodes Chapter IV deals with the use of preanodized screen printed carbon electrode (SPCE *) for the determination of lincomycin. Detection of lincomycin with a lower detection potential to enhance the effect of signal detection is achieved with SPCE*. The proposed electrochemical sensor is then coupled with techniques like solid-phase extraction and high-performance liquid chromatography for the further analysis of lincomycin in real samples such as feed, milk, honey, and urine. The uses of two types of screen printed silver electrodes are extensively studied in chapter V. One type of electrode consists of an electrode strip with silver as working electrode, AgSPE and another one with the combination of all 3 electrodes with silver as working, counter and reference electrodes in a single strip, SP-AgES. The use of SP-AgES with combination of linear sweep voltammetry explored for halide ion determination (Cl-, Br-, and I-). SP-AgES with stable reference electrode can avoid the general electrochemical disadvantage while using Ag / AgCl (3 M KCl) reference electrode such as the risk of leakage of chloride ions during the quantitative analysis of halide ions. AgSPE is used for the determination of heavy metal like mercury (Hg) in the presence of potassium iodide. And this approach was further utilized to analyze hidden Hg in the cosmetic samples. Real sample assays were consistent with that of result obtained from ICP-OES, which confirm the applicability of the proposed method for practical application. Overall in this doctoral research thesis a low cost and easy useable electrochemical detection system based on different types of screen printing electrodes combined with different analytical techniques are successfully applied for identification and detection of biologically and environmentally important sample of interest.