Influence of cell size and seedling age on seedling quality and plant growth and crops model establish of Pak-Choi ( Brassica campestris L. ssp. chinensis )

• Main Authors: Wei-Ru Kuo, 郭濰如
• Other Authors: 張武男
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/19607409983705903195

博士 === 國立中興大學 === 園藝學系所 === 97 === Chapter 2 The studies are focus on the influence of cell size and seedling age on growth of seedlings and transplants of Pak-Choi ( Brassica campestris L. ssp. chinensis (L.)) in plug system. The `Feng-jing'' Pak-Choi is supplied to the material in the fall and winter crops. Seedling grows in the cell size as 60, 20 and 8.5 ml of 72, 128 and 288 cells tray respectively. Transplanting at 9, 12, 15 days of 3 kinds of seedling ages. The growths of Pak-Choi seedlings become with the cell size are being related. Upside the fresh weight difference increases along with the sowing seeds time enlarges gradually. And the winter crops performance compares the fall crops. In fall crops, the fresh shoot weights of 288 cells seedling are smaller significantly sowing the 6th day later. Then sowing the 12th day later, the fresh weigh also reach the significance difference standard between the 72-and128 cells seedlings. The winter crops also has the similar result, after the sowing seeds, before, although 9 days, 3 kind of trays has not reached the remarkable difference, but the 288 cells seedling has small tendency. Then sowing the 12th day later, also by 72 cells seedling are the biggest, 128 cells seedling next best, 288 cells seedling are smallest significantly. The relative growth rate increases along with the seedling sowing date number reduces gradually, and becomes with cell size is being related. The seedling root growth also by grows seedlings in the big cell size has the remarkable more root fresh weight, and along with the growing seedlings time, the difference gradually enhances. The cell size and seedling age has the remarkable influence on the growths of Pak-Choi transplants, and has the good growths by the winter crops. In fall crops, the shoot fresh weight of transplants become is being related with the cell size and seedling age respectively. The 72 and 128 cells seedling of seedling age 15 days, upside the fresh weight may reach above 30g after transplanting 15 days later, and the 72 cells seedling transplant is the heaviest significantly. When the 18th day after transplanting, the 12 and 15 day of seedling age combination with 72 and 128 cells seedling, the fresh weight all may above 30g, and seedling age 15 day of 72 cells seedling is the heaviest, but with same seedling age of 12 cells seedling transplant has not significance difference. In winter crops, the 12 and 15 day seedling age of 72 cells seedling transplants, the fresh weight both may above 30 g after transplanting 15 days, but between seedling age 15 day of 72 and 128 cells seedling transplants has not significance difference. After transplanting 18 days, all treatment may above 30 g but seedling age 9 day of 288 cells seedling transplants. Both of fall and winter crops, the relative growth rate along with the cultivation date number increases, but drops by seedling age gradually. The smaller transplanting seedling age has the higher relative growth rate, and the fall crops compares with the winter crops to be low. In transplanting initial period of fall and winter crops, the relative growth rate with cell size to become is being related, but different has the difference transplanting 9 days later. Chapter 3 In order to find the physiology reasons of variety growth in difference plug seedlings and transplants. The `Feng-jing'' Pak-Choi is supplied to the material in the fall and winter crops. The cell size as 60, 20 and 8.5 ml of 72, 128 and 288 cells tray use for grows seedlings respectively. Transplanting at12, 15, 18 days of 3 kinds of seedling ages. Compare the physiological status of seedlings and transplants. Chlorophyll content of the seedling with sowing cells size to become is being related. In fall crops, the difference between sowing cells size is more remarkable. In 27 days of growing seedlings period, the chlorophyll content assumes the slight drop on 72 and 128 cells seedlings both, and the 288 cells seedling has significance reduce sowing 15th day later. In winter crops, the difference of chlorophyll content of seedling is not remarkable between sowing cell size, and in sowing seeds to 21 day of before slight rise, and then reduces. The photosynthesis rate is high in the winter crops, with grows seedlings cell size to be related, and drops gradually. In root activity, 72 cells seedling increases along with the sowing date number rises gradually, 128 and 288 cells seedling will sowing for 18 days later to drop, and will be remarkable by 288 cells seedling in fall crops. In winter crop, the root activity of 3 kinds of cells seedlings are rise after sowing 27 days gradually, and will sowing for 18 days later with the cell size to become the inverse correlation. In root respiration rate, the fall crops are higher than winter crops. In fall grows seedling, Sowing latter 18 days ago with grows seedlings the cell size to become the inverse correlation, and 288 cells seedling will sowing for 18 days later gradually to reduce. In winter crops, all assumes the trend of escalation in 3 kind cell size seedlings. After the sowing initial period by 288 cells seedling is the highest and 72 cells seedling is the lowest significantly. But 128 and 288 cells seedlings raise rate with the growing date number slow down gradually. The 288 cells seedlings have remarkable reduce degree, but sowing 24 days later the difference is no longer remarkable. The ABA content of seedling root after sowing 15th day, the 288 cells seedling root is the highest, and the 72 cells seedling is the lowest respectively. The C2H4 liberation rate increases along with the sowing time rises in seedling root. Sowing 12 days later, the 288 cells seedling remarkable highest, the 72 and 128 cells seedlings does not have the difference. In fall crops, the chlorophyll content reduces along with the transplanting date number increases. The reduce degree has with cell size of grows seedlings e to become the inverse correlation, but becomes tendency of the related with the seedling age. In winter crops, the chlorophyll content all assumes tendency of the rise after transplanting, but the 12 day of seedling age of transplant is not remarkable. The photosynthesis rate are reduce after transplanting gradually in fall and winter crops both, and the even seedling age becomes the inverse correlation. The photosynthesis rate of fall crops is lower than the winter crops, the transplant of seedling age 12 day reduce obviously, the transplant of seedling age 18 day maintains lower degree, but transplant of 72 and 128 cells seedling transplanting 6 days later has tendency of the rise. In winter crop, the reduce degree of photosynthesis rate compares the fall crops to do not obviously. Between the cell size, the difference of photosynthesis rate is not significance, but has tendency of the related with the cell size becomes. The root activity is lower in fall crops, becomes with the seedling age is being related, and increases along with the transplanting date gradually, but the difference is not remarkable between cell size of grow seedling. After transplanting initial period, the respiration rate of transplants root and the seedling age become are being related. In fall crops, the respiration rate of transplants root is higher compare to winter crops, and becomes the trend of escalation after transplanting, but reduce obviously transplanting 6 day later. The respiration rate of transplants root to be rise gradually does not have occurrence reduce in winter crops. Chapter 4 `Feng-jing '' Pak-choi seeds are sowing in 72, 128, 288 cells tray to grow seedlings. The total ascorbate content of leaf and root of 288 cells seedling both are the highest significantly after sowing 15days. The ascorbate and dehydroascorbate content is close and along with grows seedlings cells size to enhance reduces in the leaf. The ascorbate and dehydroascorbate content of 72 cells size seedling both are the lowest significantly in the root. The total glutathione content of leaf and root with grows seedlings cells size to become is being related. The total glutathione content of leaf of 72 cells seedling is higher significantly, but the difference between 128 and 288 cells seedling is not remarkable. The 72 and 128 cells seedling total glutathione content is higher than the 288 cells seedling obviously in the root, but between 72 and 128 cells seedling difference is not remarkable. In the SOD activity, the difference of leaf and root both are not significance between 3 kinds cells seedling, but has tendency of along with grows seedlings cell size gain to enhance in the leaf, along with that reduce in the root. In the CAT activity, the difference is not significance between 72 and 128 cells seedling, but the 288 cells seedling is lower significantly in the leaf. The CAT activity of root, there are not remarkable difference between the cell size, but has along with grows seedlings cell size gain to reduce the tendency. The difference of APX activity of leaf and root both do not reach the significance level, but tendency of being related for the grows seedlings cell size in leaf, for the inverse correlation in root. The GR activity of leaf of 288 cells seedling is lower than 72 and 128 cells seedling significantly, between 72 and 128 cells seedling difference is not remarkable. In the root, that with grows seedlings cell size to become is being related remarkable, the difference reaches the significance level. Chapter 5 The Pak-Choi grows seedlings for 15 days in 128 cells tray. The seedling is treated with the brassinolide of concentration 0.1-0.05 mg L-1 before transplanting 3 days. The result to show that has the remarkable influence on growth of transplanting Pak-Choi, finally demonstrated that has the different effect in the different crops season, and may promote under water stress. In October crops, the fresh shoot weight of control is lower significantly, but has not significance difference between 0.1-0.05 mg L-1 of brassinolide treatment after transplanting 7 or 14 days. But the single fresh shoot weight of 2 treatments and control difference not to be remarkable after transplanting 21 days. In November crops, the seedling and to transplanting latter 21days transplants of 2 brassinolide concentration treatments and control difference all not to be significance. In December crops, the result Is opposite with October corps in transplanting latter 21 days, the fresh shoot weight of control is the heaviest significantly, and has not remarkable difference between 2 brassinolide concentration treatments. Similarly processing with grows seedlings the way, the seedling are transplanted into the difference water status soil medium, and investigate at transplanting 14 days latter. In water potential of soil maintain at 0 MPa crops, the 2 brassinolide concentration treatments transplants all are higher than control on fresh shoot weight significantly, but is not remarkable difference between 2 treatments. The dry shoot weight of 2 brassinolide concentration treatments and control difference all not to be significance. The soil water potential maintain at -0.04 to -0.02 MPa, the 2 brassinolide concentration treatments and control transplants difference all not to be significance on fresh and dry shoot weight respectively, but 0.1 mg L-1 of brassinolide treats transplant has slightly high fresh weight of the tendency. In the dry soil of water potential maintain at -1.38 to -0.86 MPa cultivates, the fresh weight and the dry weight of the 2 treatments are higher than the control significantly, between treatments the difference is not remarkable, and the fresh weight of 0.05 mg L-1 treatment has the slightly high tendency by density. Chapter 6 The Pak-Choi grows seedlings in 60, 20 and 8.5 ml cell size of 72, 128 and 288 cells tray and transplanting at 9, 12, 15 days of 3 kinds of seedling ages respectively. Investigate the growth of shoot per 3 day after transplanting. Analyzes the seedling age and cells size from the obtained data to growth the influence separately. And using the polynomial regression discussion growth tendency, takes basis of the establishment production model. The result showed that the growth and the seedling age and grow seedlings cells size to assume are being related significantly in seedling age 9-15 of Pak-Choi after transplanting, In the transplanting initial period, large cells size seedling is also large to the absolute and the relative growth rate, but the relative growth rate assumes the inverse correlation with the seedling age. Based on the influence on the growth, absolute growth rate and relative growth rate of seedling age and cells size before transplanting. The recommendation is when Pak-Choi transplanting after grows seedlings in plug system for take reduces the field cultivation date as the goal, may benefit 72 cells tray to grow seedlings for 15 days, the fresh weights of shoot may reach 30g after transplanting 14 days. After the disaster when rapid production recovery, grows seedlings for 9 days by 72 cells tray, the transplant fresh weight may reach the harvest standard after transplanting 19 days. Grows seedlings for 15 days by 128 cells tray, and harvest after transplanting 18 day, may give dual attention to the production benefit and the output. The pattern is the best crops model for Pak-Choi transplanting after grows seedlings in plug system.