| Summary: | 碩士 === 國立成功大學 === 口腔醫學研究所 === 97 === In 1970, it has been found that coral is a good biomaterial for bone graft, so we take nature coral as the substrate for bone repair. We modify the surface of corals by using bioabsorbable materials, chitosan, which has the good biocompatibility. Chitosan is a polysaccharide polymer which can improve the surface property by coating on many other materials. In other studies, chitosan can also be used in drug carrier in control release system, moreover, chitosan could promote alkaline phosphatase activity of osteoblast, so that it can increase the mineralization and bone regeneration. So as to achieve osteoinductivity, Purmorphamine which has been discovered in stem cell research can induce progenitor mesenchymal stem cell differentiation into osteoblast. Therefore, the aim of this study is to use chitosan carrying Purmorphamine to reach bone repair on drug delivery system. First we culture bone marrow cells on the chitosan-coral composite to observe cell morphology and evaluate the ability of cell proliferation and differentiation in vitro. In vivo, we also implanted the chitosan-coral composite in the bone defect of the rat cranial bone in order to evaluate the bone regeneration and repair. Using above methods to modify the composites can make them have both osteoconductive and osteoinductive , so that the composites would be an ideal scaffold for bone graft and repair.
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