| Summary: | 碩士 === 國立成功大學 === 工程科學系碩博士班 === 97 === In this study, the Polydimethylsilcoxane (PDMS) concentration gradient microfluidic chip and PDMS stamp are fabricated by MEMS, casting molding, and microimprinting technology. The chip is used to research optimizing concentration of endothelial cells on glucose concentration gradient. The experiment strategy is based on a microfluidic network to generate a concentration gradient in solution, and observes the effect of concentration gradient in different flow rates. Besides, it combines micropatterns that cell adhesion space width with 300 μm and gap with 200 μm to discuss the optimizing glucose concentration (0~10%, 0~20%, 5~15%, 6~17%) of endothelial cells. Through the experiment data, we can prove that the concentration gradients are all stable under different flow rates (20, 30, 40, 50 μL/min), however, when flow rates are over 20 μL/min, cell leaves substrate surface. The result shows that the glucose concentration gradient in the 7~15% is the optimizing concentration of endothelial cells, nevertheless, too high and low concentration will lead cell to leave or die. This study can solve the problems of the different drug concentrations prepare and test. It will apply drug concentration test and hypersensitive test in the future.
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