| Summary: | 碩士 === 國立成功大學 === 化學工程學系碩博士班 === 97 === Biohydrogen from cellulosic feedstock has been considered a promising and economical route of producing bioenergy. Converting inert cellulosic materials to fermentable feedstock via pretreatment/hydrolysis is the key technology towards efficient bioenergy production. In this work, pure (carboxymethyl-cellulose (CMC) and xylan) and natural (bagasse) cellulosic materials were first pretreated by acid and alkaline reagents, and was then hydrolyzed by extracellular cellulase/xylanase produced from an isolated Cellulomonas uda E3-01 strain. A temperature-shift strategy (35oC for cellulolytic enzymes production and 45oC for hydrolysis reaction) was used to increase the reducing sugar (especially, monosaccharide and disaccharide) concentration in the hydrolysates. The CMC, xylan, and bagasse hydrolysates were successfully converted to H2 via dark fermentation with Clostridium butyricum CGS5, attaining a maximum hydrogen yield of 8.8 mmol H2/g reducing sugar (7.9 mmol H2/g substrate), 6.0 mmol H2/g reducing sugar (5.4 mmol H2/g substrate), and 6.0 mmol H2/g reducing sugar (5.4 mmol H2/g substrate).
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