Creation of Nano-liter Droplets for Single-Particle Trapping Utilizing Negative Dielectrophoresis and Liquid Dielectrophoresis

• Main Authors: Sung-Lin Tsai, 蔡松霖
• Other Authors: Ling-Sheng Jang
• Format: Others
• Language: en_US
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/04625729517637945829

碩士 === 國立成功大學 === 奈米科技暨微系統工程研究所 === 97 === In recent ten years, Micro-Electro-Mechanical Systems (MEMS) technology is established and is more efficient rather than the conventional ones. dielectrophoresis (DEP) is one of the electrical methods to manipulate μ-particles, and can be easily combined with subsequent analyses based on electric fields. In 1951, DEP was first defined as polarizable particles moving into regions of higher electric field intensity. Besides, negative DEP is practicable in trapping particles suspended in the liquid. In contrast to DEP, liquid DEP (LDEP) is a particular case of DEP. The phenomenology of liquid DEP is that the dielectric liquid tends to flow towards to the regions of high electric field intensity under a non-uniform electric field. Additionally, the relationship between DEP and LDEP is discussed experimentally and theoretically. Furthermore, this research presents a method to create a 20 nl droplet of deionized water by LDEP. Finally, the creation of a water droplet containing 10 μm and 15 μm polystyrene beads at the desired location from the continuous flow driven by LDEP is successfully demonstrated using the developed method. This work presents a practical method of DEP and LDEP based on parallel electrodes for single particle trapping application.