| Summary: | 碩士 === 國立暨南國際大學 === 生物醫學科技研究所 === 97 === Recently, the nano-metal structures that can produce some interested near-optical properties have been widely and deeply studied. In our work, we used two methods , nanosphere lithography and chemical reduction, to fabricate nano-metal structure ans study their localize surface plasmon resonance (LSPR) by characterizing the absorption spectra. Since the LSPR is sensitive to the refractive index modulation, we further proved our fabricated nano-metal structure could be used as a DNA sensor.
Nanosphere lithography has to use a self-assembly technique to generate a mono-layered and nano-sized polystyrene (PS) thin film which serves as a mask for fabricating the nano-metal structure. However, due to the limitation of etching equipment we were unable to successfully fabricate the nano-metal structure. After a long effort, we changed the fabrication procedure by using a chemical reducation method and a particle size of about 10 ~ 15nm nanometer gold particle solution was successfully fabricated. In the experiment, we compared the optical properties of LSPR in the nano-gold solution which could be fabricated either in the water phase or the organic phase. The gold nanoparticles were self-assembled in the glass substrate which was expected to make a microfluidic channel device for DNA sensing. The resurts indicated a successful DNA sensor which has a detectable wavelength shift of DNA signal from 100 nM target DNA.
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