Cloning and tissue expression of peroxisome proliferator-activated receptor α in Asian seabass, Lates calcarifer

• Main Authors: Shu-Ting Hsu, 許淑婷
• Other Authors: Su-Mei Chen
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/45010093003193643172

碩士 === 國立嘉義大學 === 水生生物科學系研究所 === 97 === Peroxisome proliferator activated receptors (PPARs) are a family of hormone receptors implicated in a collection of fundamental biological processes, mainly related to lipid metabolism regulation. Three subtypes of PPAR, termed α, β and γ have been identified in several vertebrate species. The aim of present study was to �� clone the PPARα gene and �� examine the gene expression of PPARα in various tissues and the related with body composition in different body size of Asian seabass (Lates calcarifer). �� A 256-bp cDNA fragment of PPARα was cloned from Asian seabass by reverse transcription polymerase chain reaction (RT-PCR) using specific oligonucleotide primers which were designed according to known evolutionary conserved sequences of certain PPARα domains, available at NCBI. A comparison between the correspondent PPARα cDNA sequences of the Japanese seaperch (Lateolabrax japonicus), red seabream (Pagrus major), Japanese pufferfish (Takifugu rubripes), house mouse (Mus musculus) and human (Homo sapiens) revealed 93%, 92%, 87%, 79% and 77% identity, respectively. �� Expression of PPARα gene in various tissues and different body size ranged from 3.34 g to 8.03 g and 618 g of Asian seabass were analyzed. Expression of PPARα gene in 3.34 g to 8.03 g of juvenile Asian seabass was detected in the liver, spleen, heart, kidney, brain, pyloric caeca, intestine, dorsal muscle and gill. It showed abundant in the pyloric caeca and intestine, and at a barely detectable level in spleen and gill. Expression of PPARα gene in marketing size (618 g) of Asian seabass was detected in the liver, spleen, heart, head kidney, trunk kidney, brain, pyloric caeca, intestine, dorsal muscle, ventral muscle and gill. It showed abundant in trunk kidney, spleen, heart and brain, and at a lower level in liver, dorsal muscle, ventral muscle and gill. Proximate analyses showed that a negative correlation between the crude lipid level, hepatosomatic index and body weight was found (p<0.05). In conclusion, the 256 bp cDNA fragment of PPARα in Lates calcarifer has been cloned. The highest expression of PPARα gene was found in pyloric caeca and intestine in juvenile Lates calcarifer with body weight of 3.34 g to 8.03 g. It showed abundant in spleen, heart, trunk kidney and brain in marketing size (618 g) of Lates calcarifer.